ProtoScript II Reverse Transcriptase M0368S from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $99.00 Inquire
Catalog NumberM0368S
Size4000 units
ApplicationsDNA Analysis
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(201) Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeastBiotechnology for BiofuelsDecember 1, 2017Bingyin Peng, Manuel R. Plan, Alexander Carpenter, Lars K. Nielsen, Claudia E. Vickersfor firststrand cDNA synthesis in a 10 μL reaction using ProtoScript® II Reverse Transcriptase (NEB #M0368). The diluted cDNA was used as the template for quantitative real-time (qRT) PCR (primers are listed

(202) Bacterial-Chromatin Structural Proteins Regulate the Bimodal Expression of the Locus of Enterocyte Effacement (LEE) Pathogenicity Island in Enteropathogenic Escherichia colimBioSeptember 6, 2017Hervé Leh, Ahmad Khodr, Marie-Christine Bouger, Bianca Sclavi, Sylvie Rimsky, Stéphanie Bury-Monéreaction mixture volume using Moloney murine leukemia virus (M-MuLV) reverse transcriptase (catalog no. M0368S; New England BioLabs) following the manufacturer’s instructions in the presence of a mixture of reverse

(203) Transcriptome-wide measurement of translation by ribosome profilingMethodsAugust 1, 2017Nicholas J. McGlincy, Nicholas T. Ingolia(Human/Mouse/Rat),Illumina,MRZG126,– 10 mM dNTP mix,Invitrogen,18427-013,– ProtoScript II,New England Biolabs,M0368L,15 Sodium hydroxide,EMD Chemicals,SX0590-1,16 CircLigase II,Epicentre,CL9021K,17 DyNAmo HS SYBR Green qPCR Kit

(204) Inflammatory and apoptotic remodeling in autonomic nervous system following myocardial infarctionPLOS ONEMay 18, 2017Chen Gao, Kimberly Howard-Quijano, Christoph Rau, Tatsuo Takamiya, Yang Song, Kalyanam Shivkumar, Yibin Wang, Aman Mahajanfirst-strand cDNA synthesis using Random Primer and ProtoScript II Reverse Transcriptase (New England BioLabs, M0368L) according to manufacturer’s instruction. Real-time PCR (Table 1) was performed using IQ SYBR Green Supermix

(205) Members of Bitter Taste Receptor Cluster Tas2r143/Tas2r135/Tas2r126 Are Expressed in the Epithelium of Murine Airways and Other Non-gustatory TissuesFrontiers in physiologyJanuary 1, 2017Liu S, Lu S, Xu R, Atzberger A, Günther S, Wettschureck N, Offermanns S.to remove genomic DNA. First-strand cDNA was synthesized using ProtoScript II Reverse Transcriptase (M0368, New England BioLabs). Real-time qPCR primers were designed with the online tool provided by Roche,

(206) Developmental vascular remodeling defects and postnatal kidney failure in mice lacking Gpr116 (Adgrf5) and Eltd1 (Adgrl4)PloS oneJanuary 1, 2017Lu S, Liu S, Wietelmann A, Kojonazarov B, Atzberger A, Tang C, Schermuly RT, Gröne HJ, Offermanns S.Kit (74004, Qiagen). First-strand cDNA was synthesized using ProtoScript II Reverse Transcriptase (M0368, New England BioLabs). The same reaction was performed without reverse transcriptase as non-RT control

(207) A squalene synthase protein degradation method for improved sesquiterpene production in Saccharomyces cerevisiaeMetabolic EngineeringJanuary 1, 2017Bingyin Peng, Manuel R. Plan, Panagiotis Chrysanthopoulos, Mark P. Hodson, Lars K. Nielsen, Claudia E. Vickersfirst-strand cDNA synthesis in a 10 μl reaction 9 using ProtoScript® II Reverse Transcriptase (NEB #M0368). The diluted cDNA was used as the template for quantitative real-time (QRT) PCR (primers are listed

(208) Endothelial cation channel PIEZO1 controls blood pressure by mediating flow-induced ATP releaseThe Journal of clinical investigationDecember 1, 2016Wang S, Chennupati R, Kaur H, Iring A, Wettschureck N, Offermanns S.cells using Qiagen Micro Kit, and cDNA was preamplified using ProtoScript II Reverse Transcriptase (M0368S, New England BioLabs) for expression analysis by RT-PCR. Quantitative PCR primer sequences. Quantitative PCR

(209) Rhabdovirus-like endogenous viral elements in the genome of Spodoptera frugiperda insect cells are actively transcribed: Implications for adventitious virus detectionBiologicalsJuly 1, 2016Christoph Geisler, Donald L. Jarvisaccording to the manufacturer's instructions. RNA was reverse-transcribed with the ProtoScript® II RT kit (M0368, New England Biolabs) using random hexamers according to the manufacturer's instructions. 2.4. PCR amplification

(210) Identification of the Cysteine Protease Legumain as a Potential Chronic Hypoxia-Specific Multiple Myeloma Target GeneCellsJanuary 1, 0001"Clees AS, Stolp V, Häupl B, Fuhrmann DC, Wempe F, Seibert M, Weber S, Banning A, Tikkanen R, Williams R, et al."Moloney murine leukemia virus reverse transcriptase (ProtoScript II reverse transcriptase, New England Biolabs, #M0368) in a total volume of 20 μL. Real-time PCRs (Opticon 2 reader, MJ research, now BioRad, Dreieich, Germany)

(211) The CARD8 inflammasome dictates HIV/SIV pathogenesis and disease progressionCellJanuary 1, 0001"Wang Q, Clark KM, Tiwari R, Raju N, Tharp GK, Rogers J, Harris RA, Raveendran M, Bosinger SE, Burdo TH, et al.",Thermo Fisher Scientific,K1032 Recombinant Cas9 protein,IDT,1081059 ProtoScript II Reverse Transcriptase,NEB,M0368 IPTG,Sigma,I6758 Lenti-X Concentrator,TaKaRa,631232 Critical commercial assays,Critical commercial

(212) Telomere Dysfunction Induces Sirtuin Repression that Drives Telomere-Dependent DiseaseCell metabolismJanuary 1, 0001"Amano H, Chaudhury A, Rodriguez-Aguayo C, Lu L, Akhanov V, Catic A, Popov YV, Verdin E, Johnson H, Stossi F, et al.",Thermo Fisher Scientific,Cat# 15596026 DNase I,New England Biolabs,Cat# M0303 Protoscript II,New England Biolabs,Cat# M0368 SensiFAST™ Probes,Bioline,Cat# BIO-86005 SensiFAST™ SYBR Kit,Bioline,Cat# Bio-98020 Cycloheximide,Sigma,Cat#

(213) Identification of murine gammaherpesvirus 68 miRNA-mRNA hybrids reveals miRNA target conservation among gammaherpesviruses including host translation and protein modification machineryPLoS pathogensJanuary 1, 0001"Bullard WL, Kara M, Gay LA, Sethuraman S, Wang Y, Nirmalan S, Esemenli A, Feswick A, Hoffman BA, Renne R, et al."cDNA was synthesized from 1μg of total RNA using the NEB ProtoScript II Reverse Transcriptase (NEB, M0368S) per the manufacturer’s instructions. Quantification of selected genes were performed on an iCycler

Be the First to Write a Review!

ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

New England Biolabs

Citations (213)

Reviews

ProtoScript II Reverse Transcriptase from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map