ProtoScript II Reverse Transcriptase M0368S from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $99.00 Inquire
Catalog NumberM0368S
Size4000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(121) Thrombopoietin from hepatocytes promotes hematopoietic stem cell regeneration after myeloablationeLifeAugust 31, 2021Longfei Gao, Matthew Decker, Haidee Chen, Lei Dingalgorithm",Prism,GraphPad,, Other,SuperScript III,ThermoFisher,Cat# 18080093, Other,ProtoScript II,NEB,Cat# M0368S, Other,PELCO Cryo-Embedding compound,"Ted Pella, Inc","Cat# 27,300",

(122) DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stressPLOS GeneticsAugust 19, 2021Kosuke Nozawa, Jiani Chen, Jianjun Jiang, Sarah M. Leichter, Masataka Yamada, Takamasa Suzuki, Fengquan Liu, Hidetaka Ito, Xuehua Zhong(NEB; M0303) and reverse transcribed into cDNA using ProtoScript II First Strand cDNA Synthesis (NEB; M0368). Quantitative PCR was performed by CFX96 Real-Time System (Bio-Rad) using the SYBR Green Master Mix

(123) Per1/Per2–Igf2 axis–mediated circadian regulation of myogenic differentiationJournal of Cell BiologyJuly 5, 2021Nobuko Katoku-Kikyo et al.Fisher Scientific). cDNA was synthesized with ProtoScript II reverse transcription (New England Biolabs; M0368L). qPCR was performed with the primers listed in Table S1 and GoTaq qPCR Master Mix (Promega; A6002)

(124) Simultaneous detection and mutation surveillance of SARS-CoV-2 and multiple respiratory viruses by rapid field-deployable sequencingMed (N Y)June 11, 2021Chongwei Bi et al.transcription of RNA samples was done using either NEB ProtoScript II reverse transcriptase (NEB Cat. No M0368) or Invitrogen SuperScript IV reverse transcriptase (Thermo Fisher Scientific Cat. No 18090010), following

(125) Substrate deformation regulates DRM2-mediated DNA methylation in plantsScience advancesJune 1, 2021Fang J, Leichter SM, Jiang J, Biswal M, Lu J, Zhang ZM, Ren W, Zhai J, Cui Q, Zhong X, et al.microgram of total RNA was reverse-transcribed into cDNA with ProtoScript II (New England Biolabs, M0368L), followed by qPCR with SYBR Green Master Mix (Bio-Rad, 1725124) using CFX96 Real-Time System 690 (Bio-Rad).

(126) Dynamics of B cell repertoires and emergence of cross-reactive responses in patients with different severities of COVID-19Cell ReportsMay 9, 2021Zachary Montague et al.assays ,, RNeasy Mini isolation kit,QIAGEN,74106 Proto- Script® II Reverse Transcriptase kit,New England Biolabs,M0368S Phusion® High-Fidelity DNA Polymerase,New England Biolabs,M0530S QIAquick Gel Extraction Kit,QIAGEN,28704 Bac-to-Bac® Baculovirus Expression System

(127) Broad transcriptional response of the human esophageal epithelium to proton pump inhibitorsJournal of Allergy and Clinical ImmunologyMay 1, 2021Mark Rochman, Yong Mei Xie, Lydia Mack, Julie M. Caldwell, Andrea M. Klingler, Garrett A. Osswald, Nurit P. Azouz, Marc E. RothenbergcDNA synthesis was performed according to the protocol of the ProtoScript II Reverse Transcriptase kit (NEB, M0368). ELISA and multiplex Cells were treated with IL-13 at 100 ng/mL for 24 hours. Prior to collecting

(128) Chemoproteomics-enabled discovery of covalent RNF114-based degraders that mimic natural product functionCell chemical biologyApril 15, 2021Luo M, Spradlin JN, Boike L, Tong B, Brittain SM, McKenna JM, Tallarico JA, Schirle M, Maimone TJ, Nomura DK.Monarch Total RNA Miniprep Kit,New England BioLabs,T2010S ProtoScript II reverse transcriptase Kit,New England BioLabs,M0368L DyNAmo HS SYBR Green Kit,Fisher Scientific,F-410L Deposited Data,Deposited Data,Deposited Data Experimental Models

(129) A Robust, Safe, and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Wastewater SamplesGlobal ChallengesApril 1, 2021Gerardo Ramos‐Mandujano et al.transcription of RNA samples was done using either NEB ProtoScript II reverse transcriptase (NEB Cat. No M0368) or Invitrogen SuperScript IV reverse transcriptase (Thermo Fisher Scientific Cat. No 18090010), following

(130) Reprogramming of translation in yeast cells impaired for ribosome recycling favors short, efficiently translated mRNAseLifeMarch 25, 2021Swati Gaikwad, Fardin Ghobakhlou, David J Young, Jyothsna Visweswaraiah, Hongen Zhang, Alan G HinnebuschrRNAs. The rRNA depleted samples were reverse-transcribed using Protoscript II (New England Biolabs; M0368L) and subsequently circularized by CircLigase II (Epicentre; CL9025K). Each ‘circularized’ library was

(131) Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House EnzymesACS OmegaMarch 23, 2021Masateru Takahashi et al.enzymes was added to the respective reaction of MMLV-RT, ProtoScript II (New England Labs, cat. no. M0368), NEB AMV (AMV Reverse Transcriptase) (New England Labs, cat. no. M0277), Superscript II (Invitrogen,

(132) Translational adaptation of human viruses to the tissues they infectCell ReportsMarch 16, 2021Xavier Hernandez-Alias, Hannah Benisty, Martin H. Schaefer, Luis SerranoT4 Polynucleotide Kinase,New England BioLabs,Cat#M0201 ProtoScript II Reverse Transcriptase,New England BioLabs,Cat#M0368 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, miRNeasy Mini kit

(133) F-box protein CFK1 interacts with and degrades de novo DNA methyltransferase in ArabidopsisThe New phytologistMarch 1, 2021Chen J, Liu J, Jiang J, Qian S, Song J, Kabara R, Delo I, Serino G, Liu F, Hua Z, et al.with DNase I (NEB; M0303) and reverse transcribed into cDNA using First Strand cDNA Synthesis (NEB; M0368). Quantitative PCR was performed by CFX96 Real-Time System (BioRad) using Vazyme 2X ChamQ reagent for

(134) INSIGHT: A population-scale COVID-19 testing strategy combining point-of-care diagnosis with centralized high-throughput sequencingScience AdvancesFebruary 12, 2021Qianxin Wu, Chenqu Suo, Tom Brown, Tengyao Wang, Sarah A. Teichmann, Andrew R. Bassett(100 mM),Thermo FisherScientific,R0481 RNase H,NEB,M0297L ProtoScript II reversetranscriptase,NEB,M0368S T7 RNA polymerase,NEB,M0251L BSA (20 mg/ml),NEB,B9000S PCRD lateral flowimmunoassay,Abingdon Health,FG-FD51673 Qubit RNA HS Assay Kit

(135) Reverse Transcriptase: From Transcriptomics to Genome EditingTrends in BiotechnologyFebruary 1, 2021Samara Martín-Alonso, Estrella Frutos-Beltrán, Luis Menéndez-AriasII (New England Biolabs) (MLV RT) (1) New England Biolabs (https://international.neb.com/products/m0368‐protoscript‐ii‐reverse‐ transcriptase#Product%20Information) (2) Bagnoli, J.W. et al. (2018). Sensitive

(136) DNA Sensing in Mismatch Repair-Deficient Tumor Cells Is Essential for Anti-tumor ImmunityCancer cellJanuary 11, 2021Lu C, Guan J, Lu S, Jin Q, Rousseau B, Lu T, Stephens D, Zhang H, Zhu J, Yang M, et al.Inc.,https://www.flowjo.com/solutions/flowjo/downloads Other,Other,Other TRIZOL REAGENT,Thermo-Invitrogen,Cat# 15596018 ProtoScript® II Reverse Transcriptase,NEB,Cat# M0368L Dulbecco’s Modified Eagle’s Medium,Sigma- Aldrich,Cat# D6429 KEY RESOURCES TABLE

(137) Characterization of FGFR signaling in prostate cancer stem cells and inhibition via TKI treatmentOncotargetJanuary 5, 2021Ko J, Meyer AN, Haas M, Donoghue DJ.nanodrop. 100 ng of total RNA was used to prepare cDNA using ProtoScript II Reverse Transcriptase (NEB #M0368) with oligo(dT) primers (Cat# 51-01-15-01, IDT) following the manufacturer’s protocol. qPCR was performed

(138) Protocol for Disome Profiling to Survey Ribosome Collision in Humans and ZebrafishSTAR ProtocolsDecember 30, 2020Mari Mito, Yuichiro Mishima, Shintaro Iwasaki(Accessories of CL9025K),Lucigen,N/A ProtoScript II Reverse Transcriptase,New England Biolabs,Cat# M0368L 5× Protoscript II buffer (Accessories of M0368L),New England Biolabs,N/A DTT (100 mM) (Accessories

(139) CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypesScienceDecember 11, 2020Ryan Muller, Zuriah A. Meacham, Lucas Ferguson, Nicholas T. Ingoliaextraction (67). Reverse transcription was carried out using Protoscript II reverse transcriptase (NEB #M0368L) with oligo-dT priming according to manufacturer instructions. Quantitative RT-PCR was carried out using

(140) Disturbed flow-induced Gs-mediated signaling protects against endothelial inflammation and atherosclerosisJCI insightDecember 3, 2020Nakayama A, Albarrán-Juárez J, Liang G, Roquid KA, Iring A, Tonack S, Chen M, Müller OJ, Weinstein LS, Offermanns S.DNA synthesis was performed using the ProtoScript II Reverse Transcription kit (New England BioLabs, M0368S). qPCR was performed using primers designed with the online tool provided by Roche and the Light-Cycler

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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