Human CD16a / FCGR3A Protein (176 Val, His Tag) 10389-H08H1 from Sino Biological, Inc.

Product Specs
ItemHuman CD16a / FCGR3A Protein (176 Val, His Tag)
CompanySino Biological, Inc.
Price $298.00Supplier Page View Company Product Page
Catalog Number10389-H08H1
Conjugate/TagC-His
Purity> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC
TypeRecombinant protein
Molecular WeightThe secreted recombinant human CD16a consists of 203 amino acids and predicts a molecular mass of 23.3 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of human CD16a is approximately 44.2 KDa due to glycosylation
SpeciesHuman
Molecule NameFCGR3A
Source/Expression SystemHEK293 Cells
Activity1. Loaded Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) on His1K Biosensor, can bind anti-VEGFA(Research grade Bevacizumab Biosimilar) with an affinity constant of 0.83 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 2. Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) captured on CM5 Chip via Anti-his antibody chip, can bind anti-VEGFA(Research grade Bevacizumab Biosimilar) with an affinity constant of 0.31 µM as determined in an SPR assay (Biacore T200) (Routinely tested). 3. anti-VEGFA(Research grade Bevacizumab Biosimilar) captured on ProA chip, can bind Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) with an affinity constant of 0.78 µM as determined in an SPR assay (Biacore T200) (Routinely tested). 4. Loaded Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) on His1K Biosensor, can bind anti-VEGFA (Research grade Bevacizumab Biosimilar) with an affinity constant of 0.81 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 5. Loaded anti-CD20(Research grade Rituximab Biosimilar) on ProA Biosensor, can bind Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) with an affinity constant of 0.622 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 6. Loaded Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) on HIS1K Biosensor, can bind anti-CD20(Research grade Rituximab Biosimilar) with an affinity constant of 0.447 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 7.Loaded Anti-CD20 (Research Grade Rituximab Biosimilar) (Cat. No. 68055-H005) on proL Biosensor, can bind Recombinant Human CD16a/FCGR3A/Fc gamma RIIIA Protein, V176, His Tag (Cat. No. 10389-H08H1) with an affinity constant of 1.430 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 8.Recombinant Human CD16a/FCGR3A/Fc gamma RIIIA Protein (F176V), His Tag (Cat. No. 10389-H08H1) captured on CM5 chip via Anti-his antibody, can bind Anti-HER2 (Research Grade Trastuzumab Biosimilar) with an affinity constant of 0.158 µM as determined in an SPR assay (Biacore T200) (QC tested). 9.Anti-HER2 (Research Grade Trastuzumab Biosimilar) immobilized on CM5 chip, can bind Recombinant Human CD16a/FCGR3A/Fc gamma RIIIA Protein (F176V), His Tag (Cat. No. 10389-H08H1) with an affinity constant of 0.142 µM as determined in an SPR assay (Biacore 8K) (Routinely tested)
FormatLyophilized from sterile PBS, pH 7.4
NCBI Full Gene NameFc gamma receptor IIIa
NCBI Gene AliasesCD16, CD16-II, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, FcGRIIIA, IGFR3, IMD20
Size100 µg

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Rhesus Cytomegalovirus-encoded Fcγ-binding glycoproteins facilitate viral evasion from IgG-mediated humoral immunityNature communicationsJanuary 31, 2025Otero CE, Petkova S, Ebermann M, Taher H, John N, Hoffmann K, Davalos A, Moström MJ, Gilbride RM, Papen CR, et al.His-tagged FcγR (human CD16A/FcγRIIIA F176V) ectodomains were used at a final concentration of 5 µg/ml (10389-H08H1 and 90013-C27H, Sino Biological). Dead cells were labeled via DAPI stain. Analysis was performed on

(2) Systematic analysis of Fc mutations designed to reduce binding to Fc-gamma receptorsmAbsDecember 31, 2024Geoff Hale, Jelle De Vos, Alastair Douglas Davy, Koen Sandra, Ian Wilkinson(CD16a) 158F allele,human,Sino Biological,10389-H08H FcγRIIIa (CD16a) 158 V allele,human,Sino Biological,10389-H08H1 FcγRIIIb (CD16b) NA1 allele,human,Sino Biological,11046-H08H1 FcγRIIIb (CD16b) NA2 allele,human,Sino Biological

(3) Enhanced efficacy of glycoengineered rice cell-produced trastuzumabPlant biotechnology journalNovember 1, 2024Shin JH, Oh S, Jang MH, Lee SY, Min C, Eu YJ, Begum H, Kim JC, Lee GR, Oh HB, et al.resonance (SPR) The binding of TMab and P‐TMab to human FcγRIIIa variants (V158 and F158 allotypes) (10389‐H08H1 and 10389‐H08H; Sino Biological, Beijing, China) was assessed using a Biacore X100 surface plasmon resonance

(4) PvDBPII elicits multiple antibody-mediated mechanisms that reduce growth in a Plasmodium vivax challenge trialNPJ vaccinesJanuary 6, 2024Martinez FJ, White M, Guillotte-Blisnick M, Huon C, Boucharlat A, Agou F, England P, Popovici J, Hou MM, Silk SE, et al.(FcγRIa), FcγRIIa, FcγRIIb, and FcγRIIIa (all from Sino Biological: 10256-H08H, 10374-H08H1, 10259-H08H and 10389-H08H1, respectively) at dilutions 19 nM, 1.7 μM, 3.7 μM and 2.3 μM, respectively. The method was set as follows,

(5) Specific peptide conjugation to a therapeutic antibody leads to enhanced therapeutic potency and thermal stability by reduced Fc dynamicsScientific ReportsOctober 2, 2023Masato Kiyoshi, Makoto Nakakido, Abdur Rafique, Minoru Tada, Michihiko Aoyama, Yosuke Terao, Satoru Nagatoishi, Hiroko Shibata, Teruhiko Ide, Kouhei Tsumoto, et al.on the CM5 sensor chip (Cat. No. 29149603, Cytiva). The His-tagged human FcγRIIIa reagent (Cat. No. 10389-H08H1, Sino biologicals) was subsequently injected and captured on the sensor chip. The aimed capture level

(6) Production of recombinant human IgG1 Fc with beneficial N-glycosylation pattern for anti-inflammatory activity using genome-edited chickensCommunications biologyJune 1, 2023Park JS, Choi HJ, Jung KM, Lee KY, Shim JH, Park KJ, Kim YM, Han JY.Sino Biological Inc, Beijing, China (FcγRIA-10256-H08H; FcγRIIA 167 Arg-10374-H08H; FcγRIIIA F176V-10389-H08H1; FcRn-CT009-H08H; DC-SIGN-10200-H01H). rhIgG1 Fc was purified from juvenile ALB::hIgG1 Fc chicken serum.

(7) Potent and broad neutralization of SARS-CoV-2 variants of concern (VOCs) including omicron sub-lineages BA.1 and BA.2 by biparatopic human VH domainsiScienceAugust 19, 2022Chen C, Saville JW, Marti MM, Schäfer A, Cheng MH, Mannar D, Zhu X, Berezuk AM, Banerjee A, Sobolewski MD, et al.10256-H08H Recombinant FcγRIIA,Sino Biological,Cat# 10374-H08H Recombinant FcγRIIIA,Sino Biological,Cat# 10389-H08H1 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Blitz Protein

(8) Human cytomegalovirus antagonizes activation of Fcγ receptors by distinct and synergizing modes of IgG manipulationeLifeMarch 16, 2021Kolb P, Hoffmann K, Sievert A, Reinhard H, Merce-Maldonado E, Le-Trilling VTK, Halenius A, Gütle D, Hengel H.concentration of 5 mg/ml (1:50 dilution from reconstituted 0.25 mg/ml stock solution; Sino Biological, 10389-H08H1). Pre-incubation with Protein G (Rockland, Biotin conjugated) was performed prior to incubation with

(9) FcRn, but not FcγRs, drives maternal-fetal transplacental transport of human IgG antibodiesProceedings of the National Academy of Sciences of the United States of AmericaJune 9, 2020Borghi S, Bournazos S, Thulin NK, Li C, Gajewski A, Sherwood RW, Zhang S, Harris E, Jagannathan P, Wang LX, et al.Sino Biological), FcγRIIaH131 (10374-H08H1, Sino Biological), FcγRIIb (10259H08H, Sino Biological), FcγRIIIaV158 (10389-H08H1, Sino Biological), and FcγRIIIaF158 (10389-H08H, Sino Biological). The FcγRs were injected through flow

(10) Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodiesmAbsJuly 1, 2019Aoyama M, Hashii N, Tsukimura W, Osumi K, Harazono A, Tada M, Kiyoshi M, Matsuda A, Ishii-Watabe A.recombinant human FcγRs with C-terminal polyhistidine tag (Cat. No. 10256-H08H, 10374-H08H1, 10259-H08H, 10389-H08H1, and 11046-H08H, Sino Biological) was assessed as described previously.47 In all experiments, each sensorgram

(11) Erwin Uehlinger on the occasion of his 80th birthday, August 8, 1979Pathology, research and practiceDecember 1, 1979Adler CP, Sandritter W.

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Human CD16a / FCGR3A Protein (176 Val, His Tag)

Human CD16a / FCGR3A Protein (176 Val, His Tag)

Human CD16a / FCGR3A Protein (176 Val, His Tag)

Human CD16a / FCGR3A Protein (176 Val, His Tag) from Sino Biological, Inc.

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Sino Biological, Inc.

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Human CD16a / FCGR3A Protein (176 Val, His Tag) from Sino Biological, Inc.

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