Human CD64 / FCGR1A Protein (His Tag) 10256-H08H from Sino Biological, Inc.

Product Specs
ItemHuman CD64 / FCGR1A Protein (His Tag)
CompanySino Biological, Inc.
Price $298.00Supplier Page View Company Product Page
Catalog Number10256-H08H
Conjugate/TagC-His
Purity≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested)
TypeRecombinant protein
Molecular WeightThe recombinant human CD64A consists of 284 amino acids and has a predicted molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant human CD64A is approximately 45-60 kDa due to glycosylation. The molecular weight of this protein is around 38-52 kDa verified by SEC-MALS (Routinely tested)
SpeciesHuman
Molecule NameFCGR1
Source/Expression SystemHEK293 Cells
NCBI Full Gene NameFc gamma receptor Ia
NCBI Gene AliasesCD64, CD64A, FCG1, FCGR1, FCRI, FcgammaRI, IGFR1
Activity1.Immobilized Anti-CD20(Ro) Antibody (Rituximab) at 1 µg/mL (100 µL/well) can bind Recombinant Human CD64 / FCGR1A Protein (His Tag)(Cat:10256-H08H), the EC50 0.6-2.0 ng/mL. 2.Captured Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 5.5 nM as determined in an SPR assay (Biacore T200). 3.Captured Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 13.6 nM as determined in an SPR assay (Biacore T200). 4.Loaded Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 8.5 nM as determined in a BLI assay (Sartorius Octet Red384). 5.Loaded Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 4.7 nM as determined in a BLI assay (Sartorius Octet Red384). 6.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) with an affinity constant of 0.38 nM as determined in a BLI assay (Sartorius Octet Red384). 7.Loaded Recombinant Human CD64/FCGR1 Protein, His Tag (Cat. No. 10256-H08H) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 6.36 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 8.Loaded Herceptin on proA Biosensor, can bind Recombinant Human CD64/FCGR1 Protein, His Tag (Cat. No. 10256-H08H) with an affinity constant of 0.213 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested)
FormatLyophilized from sterile PBS, pH 7.4
Size100 µg

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) A longitudinal study of children identifies antibody Fc-mediated functions and antigen targets of immunity to Plasmodium vivax malariaImmunityMarch 10, 2026D. Herbert Opi, Rhea J. Longley, Eizo Takashima, Tim Spelman, Yanie Tayipto, Kael Schoffer, Jessica Brewster, Linda Reiling, Bruce D. Wines, Benson Kiniboro, et al.FcγRIII V158 ectodomain dimers,Burnet Institute,N/A Recombinant human FcγRI protein,Sino Biological,10256-H08H Purified human C1q,Merck Millipore,204876 "Streptavidin, (R-PE)",Thermo Fisher Scientific,SA10041 "Goat

(2) Human Fcγ-receptors selectively respond to C-reactive protein isoformsFrontiers in immunologyJanuary 1, 2025Henning A, Seer J, Zeller J, Peter K, Chen H, Thomé J, Kolb P, Eisenhardt SU, Hoffmann K, Hengel H.recombinant, HEK293/ECD, C-terminal polyhistidine tag: 1038-H08H1/10374-H08H1/10374-H08H/10259-H08H/10256-H08H/10389-H08C) to immobilized pCRP (US Biological Life Sciences) or IgG (human IgG1 kappa, # I5154-1MG,

(3) Systematic analysis of Fc mutations designed to reduce binding to Fc-gamma receptorsmAbsDecember 31, 2024Geoff Hale, Jelle De Vos, Alastair Douglas Davy, Koen Sandra, Ian WilkinsonReceptor,Species,Supplier,Catalogue number FcγRI (CD64),human,Sino Biological,10256-H08H FcγRIIa (CD32a) 131 R allele,human,Sino Biological,10374-H08H FcγRIIa (CD32a) 131 H allele,human,Sino Biological

(4) Human Fc gamma receptor IIIA blockade inhibits platelet destruction in a humanized murine model of ITPBlood advancesApril 23, 2024Gil Gonzalez L, Won KD, Tawhidi Z, Cummins E, Cruz-Leal Y, Tundidor Cabado Y, Sachs UJ, Norris PAA, Shan Y, Bhakta V, et al.the C-terminus were purchased from Sino Biological (Chesterbrook, PA). FcγRI (CD64, catalog number 10256-H08H), FcγRIIIA (CD16A, catalog number 10389-H27H), and FcγRIIIB (CD16B, catalog number 11046-H08C), as well

(5) PvDBPII elicits multiple antibody-mediated mechanisms that reduce growth in a Plasmodium vivax challenge trialNPJ vaccinesJanuary 6, 2024Martinez FJ, White M, Guillotte-Blisnick M, Huon C, Boucharlat A, Agou F, England P, Popovici J, Hou MM, Silk SE, et al.the Fc gamma receptor (FcγR) Ia (FcγRIa), FcγRIIa, FcγRIIb, and FcγRIIIa (all from Sino Biological: 10256-H08H, 10374-H08H1, 10259-H08H and 10389-H08H1, respectively) at dilutions 19 nM, 1.7 μM, 3.7 μM and 2.3 μM,

(6) Production of recombinant human IgG1 Fc with beneficial N-glycosylation pattern for anti-inflammatory activity using genome-edited chickensCommunications biologyJune 1, 2023Park JS, Choi HJ, Jung KM, Lee KY, Shim JH, Park KJ, Kim YM, Han JY.pharma, Yong-in, Korea. Human FcγRs were purchased from Sino Biological Inc, Beijing, China (FcγRIA-10256-H08H; FcγRIIA 167 Arg-10374-H08H; FcγRIIIA F176V-10389-H08H1; FcRn-CT009-H08H; DC-SIGN-10200-H01H). rhIgG1

(7) Potent and broad neutralization of SARS-CoV-2 variants of concern (VOCs) including omicron sub-lineages BA.1 and BA.2 by biparatopic human VH domainsiScienceAugust 19, 2022Chen C, Saville JW, Marti MM, Schäfer A, Cheng MH, Mannar D, Zhu X, Berezuk AM, Banerjee A, Sobolewski MD, et al.,Sino Biological,Cat# 10108-H05H RBD-Fc,"(Li et al., 2020)",N/A Recombinant FcγRIA,Sino Biological,Cat# 10256-H08H Recombinant FcγRIIA,Sino Biological,Cat# 10374-H08H Recombinant FcγRIIIA,Sino Biological,Cat# 10389-H08H1 ,, Critical

(8) HDX-MS and MD Simulations Provide Evidence for Stabilization of the IgG1-FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein BondsJournal of molecular biologyJanuary 30, 2022Anderson KW, Bergonzo C, Scott K, Karageorgos IL, Gallagher ES, Tayi VS, Butler M, Hudgens JW.

(9) Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the FcγRIa (CD64) ReceptorJournal of Research of the National Institute of Standards and TechnologyJune 17, 2021Kyle W. Anderson, Kerry Scott, Ioannis L. Karageorgos, Elyssia S. Gallagher, Tayi Venkata S., Michael Butler, Jeffrey W. Hudgensfrom sterile, pH 7.4, phosphate-buffered saline (PBS), was acquired from Sino Biological (Catalog# 10256-H08H, Beijing, China). aIL8hFc mAbs were expressed from CHO DP-12 clone#1934 cell line (American Type Culture

(10) Cross-reactivity of mouse IgG subclasses to human Fc gamma receptors: Antibody deglycosylation only eliminates IgG2b bindingMolecular immunologyNovember 1, 2020Temming AR, Bentlage AEH, de Taeye SW, Bosman GP, Lissenberg-Thunnissen SN, Derksen NIL, Brasser G, Mok JY, van Esch WJE, Howie HL, et al.

(11) FcRn, but not FcγRs, drives maternal-fetal transplacental transport of human IgG antibodiesProceedings of the National Academy of Sciences of the United States of AmericaJune 9, 2020Borghi S, Bournazos S, Thulin NK, Li C, Gajewski A, Sherwood RW, Zhang S, Harris E, Jagannathan P, Wang LX, et al.soluble, recombinant human FcγR ectodomains were injected as analytes. The FcγRs tested were FcγRI (10256-H08H, Sino Biological), FcγRIIaR131(10374-H08H1, Sino Biological), FcγRIIaH131 (10374-H08H1, Sino Biological),

(12) CD40 Enhances Type I Interferon Responses Downstream of CD47 Blockade, Bridging Innate and Adaptive ImmunityCancer immunology researchFebruary 1, 2020de Silva S, Fromm G, Shuptrine CW, Johannes K, Patel A, Yoo KJ, Huang K, Schreiber TH.

(13) FcγR Binding and ADCC Activity of Human IgG AllotypesFrontiers in immunologyJanuary 1, 2020de Taeye SW, Bentlage AEH, Mebius MM, Meesters JI, Lissenberg-Thunnissen S, Falck D, Sénard T, Salehi N, Wuhrer M, Schuurman J, et al.

(14) Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodiesmAbsJuly 1, 2019Aoyama M, Hashii N, Tsukimura W, Osumi K, Harazono A, Tada M, Kiyoshi M, Matsuda A, Ishii-Watabe A.glycoengineered anti-CD20 mAbs to recombinant human FcγRs with C-terminal polyhistidine tag (Cat. No. 10256-H08H, 10374-H08H1, 10259-H08H, 10389-H08H1, and 11046-H08H, Sino Biological) was assessed as described previously.47

(15) Enhanced Effector Functions Due to Antibody Defucosylation Depend on the Effector Cell Fcγ Receptor ProfileJournal of immunology (Baltimore, Md. : 1950)July 1, 2017Bruggeman CW, Dekkers G, Bentlage AEH, Treffers LW, Nagelkerke SQ, Lissenberg-Thunnissen S, Koeleman CAM, Wuhrer M, van den Berg TK, Rispens T, et al.

(16) Fc gamma receptor glycosylation modulates the binding of IgG glycoforms: a requirement for stable antibody interactionsJournal of proteome researchDecember 5, 2014Hayes JM, Frostell A, Cosgrave EF, Struwe WB, Potter O, Davey GP, Karlsson R, Anneren C, Rudd PM.

(17) Erwin Uehlinger on the occasion of his 80th birthday, August 8, 1979Pathology, research and practiceDecember 1, 1979Adler CP, Sandritter W.

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Human CD64 / FCGR1A Protein (His Tag)

Human CD64 / FCGR1A Protein (His Tag) from Sino Biological, Inc.

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Sino Biological, Inc.

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Human CD64 / FCGR1A Protein (His Tag) from Sino Biological, Inc.

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