The health of the cell is paramount in any cellular experimentation effort. Its’ parameters include viability, proliferation, and toxicity levels. Mitochondrial activity, DNA incorporation, and cell staining can determine cell viability and toxicity in response to added factors or conditions. Simple cell counting for harvesting purposes uses Trypan blue; more complex assessments for apoptosis require reagents like MTT or XTT. REDOX reactions initiated by almarBlue® quantify proliferation and cytotoxicity in eukaryotic and prokaryotic cells, reliably measured by absorbance and fluorimetric assays. Available in addition to these reagents is an assortment of the universally employed BrdU for cell cycle and proliferation profiling. These tests are compatible with single cell culture, multi-well plate formats, or tissues. Assay reagents are sorted by quantity and type for comparison of price, quality, and assay requirements.
Apoptosis is a form of highly organized and programmed cell death; it does not ...
The ApoTox-Glo Triplex Assay is a recent addition to a number of elegant kits ...
Tuesday, September 25, 2012
In a cell proliferation assay, you measure the number of cells, or the change in the proportion of ...
TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assays are a valuable method ...
The Vi-Cell XR Cell Viability Analyzer from Beckman Coulter is an automated cell counter that measures cell viability, cell concentration, and cell growth rate. The Vi-Cell XR automated cell counter is based on a trypan blue cell viability assay. ...
Thursday, December 08, 2011
Simplify apoptosis experiments with image-based analysis. Colorized data plots easily identify apoptotic and necrotic cells. Manually adjust gating with instant updates to data tables. View brightfield and fluorescent images for confirmation of ...
Kurt Langenbach Ph.D. discusses the evaluation of viral and toxin induced cytotoxicity and the activity of inhibitors.
Sunday, December 16, 2012
GE Healthcare’s IN Cell Analyzer 2200 is a high-speed, flexible, modular imaging system suitable for both investigative microscopy and automated high-...
Friday, December 14, 2012
AMSBIO’s customers are among those who are providing positive feedback on how the alvetex® 3D cell culture scaffold is opening up much more relevancy ...
Friday, February 10, 2012
A scientific team led by researchers at the University of Geneva (UNIGE) and the Charité Berlin Medical University has made a completely ...