CellTiter-Glo Luminescent Cell Viability Assay

October 11, 2017

University of South Alabama
Cancer Biology
Graduate Student

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Company:

Promega

Product Name:

CellTiter-Glo Luminescent Cell Viability Assay

Catalog Number:

G7573

This assay measures cell viability in the form of ATP present after lysing the cells. I used this assay to determine changes of cell viability in HCT-116 human colon tumor cells after treatment with the EGFR inhibitor Erlotinib.

Experimental Design and Results Summary

Application

Cell Viability Assay

Starting Material

HCT-116 human colon tumor cells

Protocol Overview

Treat cells with desired treatment. Mix the CellTiter-Glo substrate with the CellTiter-Glo buffer at a 50:50 ratio. At the reagent mixture at a 3X concentration, and incubate for 10 minutes in the dark at room temperature. Then read luminescence at 700 nm in a microplate reader.

Tips

When plating cells for the assay, plate so that the cells will be at 70-80% confluency when adding the reagent. This will give you high luminescence readout numbers for comparing treatment groups.

Results Summary

The assay showed that the EGFR inhibitor does not seem to decrease cell viability after treatment. I determined that I need to screen other compounds for decreased cell viability in HCT-116 cells.

DOI or PMID #

N/A

Additional Notes

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Summary

The Good

The assay is easy to perform, is quantitative, can be adapted to a high-throughput format.

The Bad

The assay is dependent on cell number, so there can be variation between experiments.

The Bottom Line

The CellTiter-Glo assay is an excellent way to determine cell viability after your treatment of choice. It is especially helpful for screening for compound effects on cell viability. I highly recommend.

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