Chromatography systems include all the equipment needed for sample separation, a power source and program software. Chromatography systems are found in all arenas of biotechnology, from small research laboratories up to large manufacturing plants. There are different technologies available for separation. LC or Liquid chromatography (LC) is a separation technique in which the mobile phase is a liquid, run under low pressure such as gravity or vacuum pump, or high pressure under mechanical pumps (HPLC). (GLC), is a separation technique in which the mobile phase is a gas. Liquid chromatography and gas chromatography can be done in conjunction with mass spectrometry instrumentation that identify unknown compounds, determine the isotope composition of elements in a molecule, and determine the structure of a compound by observing its fragmentation. Important considerations when selecting a chromatography system are the chemical and physical properties of the substance such as chemical reactivity, size, mass and shape and requirements of the laboratory, such as flow process, validation requirements and sample tracking.
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