Chromatography systems include all the equipment needed for sample separation, a power source and program software. Chromatography systems are found in all arenas of biotechnology, from small research laboratories up to large manufacturing plants. There are different technologies available for separation. LC or Liquid chromatography (LC) is a separation technique in which the mobile phase is a liquid, run under low pressure such as gravity or vacuum pump, or high pressure under mechanical pumps (HPLC). (GLC), is a separation technique in which the mobile phase is a gas. Liquid chromatography and gas chromatography can be done in conjunction with mass spectrometry instrumentation that identify unknown compounds, determine the isotope composition of elements in a molecule, and determine the structure of a compound by observing its fragmentation. Important considerations when selecting a chromatography system are the chemical and physical properties of the substance such as chemical reactivity, size, mass and shape and requirements of the laboratory, such as flow process, validation requirements and sample tracking.
Greater experimental consistency and improved throughput among the benefits
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Tips on resolving issues and challenges
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The FLUOstar Omega is a multi-mode microplate reader featuring BMG LABTECH's proprietary Tandem Technology. It uses an ultra-fast CCD-based spectrometer for UV/Vis absorbance and ultra-sensitive filters for detection in other modes. Unique to BMG ...
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Esco Labculture A2 (LA2 / LR2) series BSCs are full-sized, high performance, biological safety cabinets, designed to provide maximum operator and product protection, with large work zone area, optimum user ergonomics, and following features:
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This system has been used by our lab to purify everything from alpha synuclein to amyloid beta 42 to various human chaperone proteins. It is very easy to set up, and depending on the column you use, you can get very highly purified proteins.
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Protein purification, separation of native conformations of a protein (monomers, dimers, oligomers), enrichment of proteins (e.g. phosphoproteins), etc., are some of the most demanding protocols performed by biochemists in both...
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