HPLC Systems

HPLC Systems HPLC is a separation technique in which liquid is used to force the sample at high pressure through a column packed with stationary phase in order to identify and/or quantify the components. A typical HPLC system consists of a pump (consistent flow rate is key), an injector or autosampler, and at least one detector. Also, HPLC systems often use a column oven to adjust and control the temperature of the separation step. A variety of detector types are available, including UV-VIS, refractive index (RI), photo diode array, and fluorescence.

Biochemistry, biology, and the pharmaceutical industry all make use of the high resolution and fast separation possible with an HPLC system. Buyers can choose all-in-one systems, which tend to be more economical, or modular components, which ensure easier upgradeability in the future. Systems designed specifically for biomolecule separation are one area of interest. Ultra high-performance liquid chromatography, or UHPLC, is a common buzzword; the switch often requires additional method development, but can pay off in terms of throughput and speed.

HPLC Systems

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1220 Infinity HPLC System

1220 Infinity HPLC System

Agilent Technologies

  • Catalog Number: G4286B
  • Flow Rate: 0.2 – 10.0 mL/min
  • Maximum Pressure: 60 Mpa
  • Capacity: One 25-cm column
  • Detection: Double-beam photometer / 1024-element diode array detector
  • Software: OpenLAB EZChrom Edition / OpenLAB ChemStation Edition
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1260 Infinity Analytical SFC System
  • Catalog Number: G4309A
  • Flow Rate: 0.1 mL/min to 5 mL/min (settable), 1 mL to 5 mL/min recommended
  • Maximum Pressure: 60 Mpa
  • Capacity: Up to 3, with additional 2x TCCs up to 9
  • Temperature Range: Ambient -10 °C to 100 °C
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1290 Infinity HPLC System

1290 Infinity HPLC System

Agilent Technologies

  • Catalog Number: G4212A
  • Flow Rate: 2 mL/min at 1200 bar and 5 mL/min at 800 bar
  • Maximum Pressure: 120 Mpa
  • Capacity: Three 30 cm columns or many more shorter columns
  • Detection: 1290 Infinity Diode Array Detector
  • Software: OpenLAB EZChrom Edition / OpenLAB ChemStation Edition
  • Temperature Range: 10 °C below ambient - 100°C
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Agilent 1260 Infinity Bio-inert Quaternary LC
  • Catalog Number: G5611A
  • Flow Rate: 0.2 – 10.0 mL/min
  • Maximum Pressure: 60 Mpa
  • Detection: Variable Wavelength Detector / Multiple Wavelength Detector / Diode-Array Detector
  • Software: OpenLAB EZChrom Edition / OpenLAB ChemStation Edition
  • Temperature Range: 10 °C below ambient - 80 °C
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BioLogic DuoFlow Chromatography Systems
  • Catalog Number: BioLogic DuoFlow
  • Flow Rate: Varies by Model
  • Maximum Pressure: Varies by Model
  • Detection: UV / UV-Vis
  • Software: EZLogic Integration Software
  • Temperature Range: 4–40°C
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BioLogic LP Chromatography Systems
  • Catalog Number: BioLogic LP
  • Flow Rate: 0.1–40 ml/min
  • Maximum Pressure: 0.2 Mpa
  • Detection: UV Detector
  • Software: LP Data View Software
  • Temperature Range: 4–40°C
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ÄKTA ready Liquid Chromatography System

ÄKTA ready Liquid Chromatography System

GE Healthcare Life Sciences

  • Catalog Number: 28-9062-61
  • Maximum Pressure: 0.4 Mpa
  • Software: UNICORN control system
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Analytical to Semi-preparative HPLC System
  • Catalog Number: Inquire
  • Flow Rate: H1: 0.15–15 mL/min; H2: 0.3–30 mL/min
  • Maximum Pressure: 60 Mpa
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cLC Analytical to Preparative HPLC System
  • Catalog Number: Inquire
  • Flow Rate: 2.0–200 mL/min
  • Maximum Pressure: 34.5 Mpa
  • Capacity: Up to 5 columns
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PLC 2020 Purification System
  • Catalog Number: 21030000
  • Flow Rate: Varies with the Pump Head
  • Maximum Pressure: 28 Mpa
  • Detection: Dual, variable wavelength UV/VIS detection
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X-LC system

X-LC system

JASCO

  • Catalog Number: X-LC
  • Flow Rate: 50 uL/min to 2 mL/min
  • Maximum Pressure: 19,000 psi
  • Detection: Single wavelength UV-visible / 4 wavelength UV-visible / PDA / Fluorescence / Circular dichroism
  • Software: ChromNav Software
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2D HPLC for BioAnalysis (Co-Sense for BA)
  • Catalog Number: Co-Sense for BA
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2D Micro HPLC System for Proteome Analysis
  • Catalog Number: 2D HPLC for Proteome Analysis
  • Detection: LCMS, MALDI Spotting
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Discovery VP&#174; Analytical HPLC System
  • Catalog Number: DiscoveryVP Analytical
  • Flow Rate: 1 mL/min
  • Capacity: 5 Columns
  • Detection: UV / PDA detector
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Discovery VP&#174; Preparative HPLC System
  • Catalog Number: DiscoveryVP Prep
  • Flow Rate: Up to 150 mL/min
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LC-2010HT Liquid Chromatograph
  • Catalog Number: LC-2010HT
  • Flow Rate: 0.001 to 5 mL/min
  • Maximum Pressure: 35.0 Mpa
  • Capacity: 2 × 250 mm columns
  • Detection: UV Detector
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Nexera MP UHPLC System
  • Catalog Number: Nexera MP
  • Flow Rate: 1.2 mL/min
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Nexera Quaternary UHPLC
  • Catalog Number: Nexera Quaternary
  • Flow Rate: 1 mL/min
  • Maximum Pressure: 130 Mpa
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Nexera UHPLC Method Scouting
  • Catalog Number: Nexera Method Scouting
  • Maximum Pressure: 130 Mpa
  • Capacity: 1 to 6 columns
  • Detection: SPD-M20A (Semi-Micro Temperature Control Cell)
  • Software: LabSolutions Multi-PDA / CLASS-Agent Report
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  • Catalog Number: Nexera
  • Flow Rate: 0.0001–3.0000 mL/min (to 130 Mpa) / 3.0001–5.0000 mL/min (to 80 Mpa)
  • Maximum Pressure: 130 Mpa
  • Capacity: Up to 2 columns ( Depending on the Column Oven Model)
  • Detection: UV-VIS Detector / Photo Diode Array Detector / Fluorescence Detectors
  • Software: Open Solution software
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Perfinity Integrated Digestion Platform (iDP)
  • Catalog Number: Perfinity iDP
  • Software: Perfinity iDP Software
  • Applications: Assay Development / Protein Purification / Drug Development / Biomarker Discovery
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Prominence HPLC System
  • Catalog Number: LC-20A
  • Flow Rate: 0.0001 mL/min to 10.0000 mL/min
    0.0001 mL/min to 3.0000 mL/min (to 66 Mpa)
    3.0001 mL/min to 5.0000 mL/min (to 44 Mpa)
  • Maximum Pressure: 40 Mpa / 66 Mpa
  • Detection: UV-VIS Detector / PDA Detector / Fluorescence Detector
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Prominence Prep HPLC System
  • Catalog Number: Prep HPLC
  • Flow Rate: Up to 100 mL/min ( 42 Mpa) / Up to 150 mL/min (30 MPa)
  • Maximum Pressure: 42 Mpa
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  • Catalog Number: Prominence UFLC
  • Flow Rate: 0.0001 to 10.0000 mL/min
  • Maximum Pressure: 40 Mpa
  • Detection: UFLC version UV-VIS Detector
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Prominence UFLCXR (Ultra Fast LC w/ Extra Resolution)
  • Catalog Number: Prominence UFLCXR
  • Flow Rate: 0.0001 - 3 mL/min (1.0 to 66 Mpa) / 3.0001 - 5 mL/min (1.0 to 44 MPa)
  • Maximum Pressure: 66 Mpa
  • Detection: SPD-20A UV detector
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  • Agilent Technologies
  • Bio-Rad
  • GE Healthcare Life Sciences
  • Gilson, Inc
  • JASCO
  • Shimadzu