HPLC is a separation technique in which liquid is used to force the sample at high pressure through a column packed with stationary phase in order to identify and/or quantify the components. A typical HPLC system consists of a pump (consistent flow rate is key), an injector or autosampler, and at least one detector, such as UV-VIS, refractive index, diode array, or fluorescence. A column oven is often used for temperature adjustment. Biochemistry, biology, and the pharmaceutical industry all make use of the high resolution and fast separation possible with HPLC. Buyers can choose all-in-one systems, which tend to be more economical, or modular components, which ensure easier upgradeability in the future. Systems designed specifically for biomolecule separation are one area of interest. Ultra high-performance liquid chromatography, or UHPLC, is a common buzzword; the switch often requires additional method development, but can pay off in terms of throughput and speed.
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- Catalog Number: G4286B
- Flow Rate: 0.2 – 10.0 mL/min
- Maximum Pressure: 60 Mpa
- Capacity: One 25-cm column
- Detection: Double-beam photometer / 1024-element diode array detector
- Catalog Number: G4309A
- Flow Rate: 0.1 mL/min to 5 mL/min (settable), 1 mL to 5 mL/min recommended
- Maximum Pressure: 60 Mpa
- Capacity: Up to 3, with additional 2x TCCs up to 9
- Temperature Range: Ambient -10 °C to 100 °C
- Catalog Number: 1260 Infinity
- Flow Rate: 0.2 - 10.0 mL/minute
- Maximum Pressure: 60 Mpa
- Capacity: 0.1 - 100 µL (Can Increase Up to 1500 µL)
- Detection: 1260 Infinity Refractive Index Detector
- Temperature Range: 10 °C below ambient - 80 °C
- Catalog Number: G4212A
- Flow Rate: 2 mL/min at 1200 bar and 5 mL/min at 800 bar\
- Maximum Pressure: 120 Mpa
- Capacity: Three 30 cm columns or many more shorter columns
- Detection: 1290 Infinity Diode Array Detector
- Temperature Range: 10 °C below ambient - 100°C
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- Catalog Number: 390-MDS
- Detection: Viscometer / Light Scattering Detector
- Catalog Number: 9710000600
- Flow Rate: 0.001 to 9.999 mL/min
- Maximum Pressure: 39.2 MPa
- Capacity: Up to two columns from 4.6 to 13 mm ID and 50 to 300 mm length
- Detection: UV-Vis detector / PDA detector / Fluorescence detector (optional) / RI detector (optional) / ELS detector (external, optional)
- Temperature Range: Ambient+10 to 65 °C (90 °C option available)
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- Catalog Number: G5611A
- Flow Rate: 0.2 – 10.0 mL/min
- Maximum Pressure: 60 Mpa
- Detection: Variable Wavelength Detector / Multiple Wavelength Detector / Diode-Array Detector
- Temperature Range: 10 °C below ambient - 80 °C
- Catalog Number: BioLogic DuoFlow
- Flow Rate: Varies by Model
- Maximum Pressure: Varies by Model
- Detection: UV / UV-Vis
- Temperature Range: 4–40°C
- Catalog Number: BioLogic LP
- Flow Rate: 0.1–40 ml/min
- Maximum Pressure: 0.2 Mpa
- Detection: UV Detector
- Temperature Range: 4–40°C
GE Healthcare, Life Sciences
- Catalog Number: 28-9062-61
- Maximum Pressure: 0.4 Mpa
GE Healthcare, Life Sciences
- Catalog Number: 28-9763-37
- Flow Rate: 0.001 -25 ml/min
- Maximum Pressure: 20 Mpa
- Detection: Multiple wave-length UV detector
GE Healthcare, Life Sciences
- Catalog Number: ÄKTAexplorer
- Flow Rate: 10 mL/min / 100 mL/min
- Maximum Pressure: 10 Mpa / 25 Mpa
- Detection: Monitor UV-900
GE Healthcare, Life Sciences
- Catalog Number: 18-1900-26
- Flow Rate: 0.05-20 ml/min
- Maximum Pressure: 5 Mpa
- Detection: Monitor UPC-900 ( for UV)
GE Healthcare, Life Sciences
- Catalog Number: 11-0013-13
- Flow Rate: 0.1 -50 ml/min
- Maximum Pressure: 1 Mpa
- Detection: UV detector
GE Healthcare, Life Sciences
- Catalog Number: ÄKTAprocess
- Flow Rate: Up to 1800 l/h for large-volume manufacturing
- Detection: Single or multiple wave-length UV detector
- Catalog Number: Inquire
- Flow Rate: H1: 0.15–15 mL/min; H2: 0.3–30 mL/min
- Maximum Pressure: 60 Mpa
- Catalog Number: Inquire
- Flow Rate: 2.0–200 mL/min
- Maximum Pressure: 34.5 Mpa
- Capacity: Up to 5 columns
- Catalog Number: 21030000
- Flow Rate: Varies with the Pump Head
- Maximum Pressure: 28 Mpa
- Detection: Dual, variable wavelength UV/VIS detection
- Catalog Number: X-LC
- Flow Rate: 50 uL/min to 2 mL/min
- Maximum Pressure: 19,000 psi
- Detection: Single wavelength UV-visible / 4 wavelength UV-visible / PDA / Fluorescence / Circular dichroism
- Catalog Number: Co-Sense for BA
- Catalog Number: 2D HPLC for Proteome Analysis
- Detection: LCMS, MALDI Spotting
- Catalog Number: DiscoveryVP Analytical
- Flow Rate: 1 mL/min
- Capacity: 5 Columns
- Detection: UV / PDA detector
- Catalog Number: DiscoveryVP Prep
- Flow Rate: Up to 150 mL/min
- Catalog Number: LC-2010HT
- Flow Rate: 0.001 to 5 mL/min
- Maximum Pressure: 35.0 Mpa
- Capacity: 2 × 250 mm columns
- Detection: UV Detector
- Catalog Number: Multiplexed HPLC
- Capacity: Up to 4 columns.
- Catalog Number: Nexera MP
- Flow Rate: 1.2 mL/min
- Catalog Number: Nexera Quaternary
- Flow Rate: 1 mL/min
- Maximum Pressure: 130 Mpa
- Catalog Number: Nexera Method Scouting
- Flow Rate: 0.6 mL/min
0.5 mL/min
1.0 mL/min - Maximum Pressure: 130 Mpa
- Capacity: 1 to 6 columns
- Detection: SPD-M20A (Semi-Micro Temperature Control Cell)
- Catalog Number: Nexera
- Flow Rate: 0.0001–3.0000 mL/min (to 130 Mpa) / 3.0001–5.0000 mL/min (to 80 Mpa)
- Maximum Pressure: 130 Mpa
- Capacity: Up to 2 columns ( Depending on the Column Oven Model)
- Detection: UV-VIS Detector / Photo Diode Array Detector / Fluorescence Detectors
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- Catalog Number: Perfinity iDP
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