Chromatography is a technique for separating complex mixtures in order to purify or analyze samples such as proteins, peptides, blood components, and small molecules. The process involves a stationary phase that temporarily holds certain molecules of the mixture, which are carried along by a mobile phase—generally a liquid solvent. These chromatography solvents serve to extract, dissolve, and move the samples without permanently changing their chemical structure, making these reagents a vital component of standard separation techniques. Solvents are often used as a mixed solution in water or in combination with another solvent. Some solvents, however, are not miscible and are used as a pure reagent. Polar solvents such as water will only dissolve in other polar solvents. Common liquid solvents, such as water, methanol, isopropanol, acetonitrile and formic acid, are staple reagents in fast protein liquid chromatography (FPLC), high-performance liquid chromatography (HPLC), and liquid chromatography-mass spectrometry (LC-MS). For any given application, the ideal solvent or solution will depend on the type of chromatography, stationary phase, purity, and target molecules. Browse our product catalogs to find solvents listed by various manufacturers.
Experts share wisdom on selection criteria
read more
Expert tips for optimizing workflows
read more
The Sapphire FL Biomolecular Imager is a field-upgradable laser scanner, with a wide selection of user-adjustable lasers and filters. It features 5-micron resolution, adjustable Z-plane focus and a wide field of view of 25cm X 25cm. Applications ...
read more
Esco Airstream A2 (AC2 / AR2) series BSCs are compact-sized, low height, low energy, low noise biosafety cabinets, condensing the advanced safety and ergonomic features into a small footprint, and following features:
- The most energy efficient BSC
...
read more
I routinely use CRISPR/Cas9-based methodology to generate gene knockouts in cell lines. For this purpose, I use a ribonucleoprotein (RNP) strategy with SpCas9 purified in-house. Previously, I purified His-tagged SpCas9; this time, I wanted to test a ...
read more
The Superdex 200 Increase is a column made from agarose-dextran that enables size-dependent separation of proteins. It effectively separates proteins based on their size and offers excellent resolution specifically in the 100-300 kDa range. This ...
read more