The Biocompare Cells and Microorganisms search tool is a diverse catalog of primary cells, cell lines, and microbial strains from many suppliers. Cell lines include stable immortalized lines, cells overexpressing target genes, hybridomas, reporter cell lines, and knockout cells. It features cells from various lineages, including myeloid and lymphoid cells, neuronal cells, fibroblasts, endothelial cells, hepatocytes, myocytes, and more. Users can also search for specific cells based on tissue type or host species, providing flexibility for a wide array of research applications. Important considerations when choosing cells for research include cell line authentication, culturing requirements, genetic or phenotypic modifications, and the intended downstream application. Inspect the product datasheets from the cell supplier pages for more detailed information.
Mononuclear cells refer to blood cells that have a single, round nucleus, such as lymphocytes and monocytes. When isolated from circulating blood, they are called peripheral blood mononuclear cells (PBMC), but other sources exist, such as the umbilical cord, spleen, and bone marrow. The established method for separating mononuclear cells from blood is through density gradient centrifugation using the polysaccharide, Ficoll. Upon spinning, the cells collect in a layer called the buffy coat, which comprise about 1% of the total sample volume. From this fraction, more specific cell types can be further isolated by purification methods that target specific cell surface proteins: CD4 for T helper cells, CD8 for cytotoxic T cells, CD19 for B cells, CD14/CD16 for monocytes, among others. Mononuclear cells have been essential in the research areas of disease, therapeutics, vaccines, immunology, diagnostics and sequencing.