CTLL-2 from ATCC

Product Specs
ItemCTLL-2
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberTIB-214
TypeCell Biology
SpeciesMus musculus
Cell LineCTLL-2 T
Cell Type (standardized)Lymphocytes
Tissue (standardized)Lymph
Cell Typecytotoxic T lymphocyte
ApplicationsThe cells are dependent upon IL-2 for growth, and can be used to assay for IL-2

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) pH-responsive virus-drug conjugates for targeted PD-L1 silencing and enhanced cancer immunotherapyJournal of NanobiotechnologyMarch 30, 2026S.-Ja Tseng, Ivan M. Kempson, Yi-Cheng Ho, Pan-Chyr Yang, Zi-Xian Liao10% FBS, 1X non-essential amino acids, and 1X β-mercaptoethanol. A clone of murine CTLL-2 T cells (TIB-214, ATCC) were cultured in RPMI 1640 medium supplemented with 10% FBS, 100 U mL− 1 penicillin, 100 µg mL−

(2) IL-7/IL-15/IL-21 cytokine-fusion scaffold generates highly functional CAR T cells enriched in long-lived T memory stem cellsSci AdvMarch 13, 2026Erin B. Cole, Sara Lamcaj, Agnes V. Sydenstricker, Adilyn G. Voss, Christopher R. Hiner, Hong B. Hur, Manoj Kandpal, Natalia Valderrama Pena, Jian Hua Zheng, Ying Xiong, et al.assessed using proliferation assays on IL-7– (2B8) (Sigma-Aldrich), IL-15– (CTLL-2) (ATCC, reference no. TIB-214), and IL-21– (B9) (Sigma-Aldrich, reference no. 12121201) dependent cell lines (32). Construction of

(3) ITK overexpression enhances T cell cytotoxicity against DLBCL through the TCR-Ca(2+)-Calcineurin-NFAT-IFN-γ pathwayScientific reportsAugust 21, 2025Liu H, Zhang M, Zhu X, Chen G.cytotoxic T lymphocyte line CTLL-2 was purchased from the American Type Culture Collection (ATCC, USA, TIB-214), and the mouse B-cell lymphoma cell line A20 was purchased from ATCC (USA, TIB-208). CTLL-2 cells were

(4) A PD-1-targeted, receptor-masked IL-2 immunocytokine that engages IL-2Rα strengthens T cell-mediated anti-tumor therapiesCell Reports MedicineOctober 15, 2024Jiaxi Wu, Nicolin Bloch, Aaron Y. Chang, Ramandeep Bhavsar, Qingqing Wang, Alison Crawford, David J. DiLillo, Kristin Vazzana, Katja Mohrs, Drew Dudgeon, et al.paper,N/A YT/STAT5-Luc/IL2RaOE/PD1KO,This paper,N/A YT/STAT5-Luc/IL2RaOE/PD1OE,This paper,N/A CTLL-2,ATCC,"Cat# TIB-214, RRID:CVCL_0227" CTLL-2/STAT3-Luc,This paper,N/A CTLL-2/STAT3-Luc/mPD1,This paper,N/A MC-38,National

(5) Tumor-Localized Interleukin-2 and Interleukin-12 Combine with Radiation Therapy to Safely Potentiate Regression of Advanced Malignant Melanoma in Pet DogsClinical Cancer ResearchSeptember 13, 2024Jordan A. Stinson, Matheus Moreno P. Barbosa, Allison Sheen, Noor Momin, Elizabeth Fink, Jordan Hampel, Kim A. Selting, Rebecca L. Kamerer, Keith L. Bailey, Karl D. Wittrup, et al.IL-12 activation assays, while collagen-binding was confirmed through ELISA. Both CTLL-2 (ATCC Cat# TIB-214, RRID:CVCL_0227) and HEK Blue IL-12 (Invivogen; RRID:CVCL_UF31) cells were cultured according to vendor

(6) Tumor-localized interleukin-2 and interleukin-12 combine with radiation therapy to safely potentiate regression of advanced malignant melanoma in pet dogsClin Cancer ResSeptember 13, 2024Jordan A. Stinson, Matheus Moreno P. Barbosa, Allison Sheen, Noor Momin, Elizabeth Fink, Jordan Hampel, Kim A. Selting, Rebecca L. Kamerer, Keith L. Bailey, K. Dane Wittrup, et al.IL-12 activation assays, while collagen-binding was confirmed through ELISA. Both CTLL-2 (ATCC Cat# TIB-214, RRID:CVCL_0227) and HEK Blue IL-12 (Invivogen; RRID:CVCL_UF31) cells were cultured according to vendor

(7) Collagen-Anchored Interleukin-2 and Interleukin-12 Safely Reprogram the Tumor Microenvironment in Canine Soft-Tissue SarcomasClinical Cancer ResearchJune 1, 2023Jordan A. Stinson, Allison Sheen, Noor Momin, Jordan Hampel, Rebecca Bernstein, Rebecca Kamerer, Bahaa Fadl-Alla, Jonathan Samuelson, Elizabeth Fink, Timothy M. Fan, et al.lines HEK293-F (Gibco; RRID:CVCL_D603), B16F10 (ATCC Cat# CRL-6475, RRID:CVCL_0159), CTLL-2 (ATCC Cat# TIB-214, RRID:CVCL_0227), and HEK Blue IL-12 (Invivogen; RRID:CVCL_UF31) were cultured according to vendor instructions.

(8) Collagen-anchored interleukin-2 and interleukin-12 safely reprogram the tumor microenvironment in canine soft tissue sarcomasClin Cancer ResJune 1, 2023Jordan A. Stinson, Allison Sheen, Noor Momin, Jordan Hampel, Rebecca Bernstein, Rebecca Kamerer, Bahaa Fadl-Alla, Jonathan Samuelson, Elizabeth Fink, Timothy M. Fan, et al.lines HEK293-F (Gibco; RRID:CVCL_D603), B16F10 (ATCC Cat# CRL-6475, RRID:CVCL_0159), CTLL-2 (ATCC Cat# TIB-214, RRID:CVCL_0227), and HEK Blue IL-12 (Invivogen; RRID:CVCL_UF31) were cultured according to vendor instructions.

(9) SON-1210 - a novel bifunctional IL-12 / IL-15 fusion protein that improves cytokine half-life, targets tumors, and enhances therapeutic efficacyFrontiers in immunologyJanuary 1, 2023Cini JK, Dexter S, Rezac DJ, McAndrew SJ, Hedou G, Brody R, Eraslan RN, Kenney RT, Mohan P.ability of hIL-15 to stimulate the proliferation of the murine cytotoxic T-cell line CTLL-2 (ATCC Cat#TIB-214). CTLL-2 cells are able to proliferate in response to either human or mouse IL-15. This proliferative

(10) Optimal target saturation of ligand-blocking anti-GITR antibody IBI37G5 dictates FcγR-independent GITR agonism and antitumor activityCell reports. MedicineJune 21, 2022Liu H, Wu W, Sun G, Chia T, Cao L, Liu X, Guan J, Fu F, Yao Y, Wu Z, et al.lines ,, HEK293T,ATCC,Cat#CRL-3216 MC38,Obiosh,Cat#HYC3401 B16F10,ATCC,Cat#CRL-6475 CT26,ATCC,Cat#CRL-2638 CHO-S,Invitrogen,Cat#R80007 CTLL-2,ATCC,Cat#TIB-214 Raji,COBIOER,Cat#CBP60272 Jurkat,genomeditech,Cat#GM-C01459 ,, Experimental models: Organisms/strains,Experimental models

(11) Design and Characterization of Novel Antibody-Cytokine Fusion Proteins Based on Interleukin-21Antibodies (Basel)March 4, 2022Cesare Di Nitto, Dario Neri, Tobias Weiss, Michael Weller, Roberto De Lucapercentage of cell viability compared with unstimulated cells. 2.4. Cell Lines CHO-S (CCL-61™), CTLL2 (TIB-214™) and F9 (CRL-1720™) cells were purchased from the ATCC. Cell lines were delivered between 2020 and

(12) Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonisteLifeMay 18, 2021Glassman CR, Su L, Majri-Morrison SS, Winkelmann H, Mo F, Li P, Pérez-Cruz M, Ho PP, Koliesnik I, Nagy N, et al.

(13) Targeted Delivery of IL2 to the Tumor Stroma Potentiates the Action of Immune Checkpoint Inhibitors by Preferential Activation of NK and CD8+ T CellsCancer Immunology ResearchApril 1, 2019Cornelia Hutmacher, Nicolás Gonzalo Núñez, Anna Rita Liuzzi, Burkhard Becher, Dario Neritumor models The CT26 colon carcinoma (CRL-2638), the F9 teratocarcinoma (CRL-170), and the CTLL-2 (TIB-214) cell lines were obtained from ATCC, expanded, and stored as cryopreserved aliquots in liquid nitrogen.

(14) Targeted delivery of IL2 to the tumor stroma potentiates the action of immune checkpoint inhibitors by preferential activation of NK and CD8+ T cellsCancer Immunol ResFebruary 19, 2019Cornelia Hutmacher, Nicolas Núñez, Anna Rita Liuzzi, Burkhard Becher, Dario Neritumor models The CT26 colon carcinoma (CRL-2638), the F9 teratocarcinoma (CRL-170), and the CTLL-2 (TIB-214) cell lines were obtained from ATCC, expanded, and stored as cryopreserved aliquots in liquid nitrogen.

(15) Minimal conformational plasticity enables TCR cross-reactivity to different MHC class II heterodimersScientific ReportsDecember 1, 2012Christopher J. Holland, Pierre J. Rizkallah, Sabrina Vollers, J. Mauricio Calvo-Calle, Florian Madura, Anna Fuller, Andrew K. Sewell, Lawrence J. Stern, Andrew Godkin, David K. ColePerkin-Elmer, Shelton, CT). IL-2 assay IL-2 in supernatants was determined by using CTLL-2 cells (TIB-214, ATCC Manassas, VA) as previously described30. Briefly, 50 μl of culture medium from stimulated HA1.7

(16) Processing And Presentation of Variant Surface Glycoprotein Molecules to T Cells In African Trypanosomiasis1The Journal of ImmunologySeptember 1, 2009Taylor R. Dagenais, Bailey E. Freeman, Karen P. Demick, Donna M. Paulnock, John M. MansfieldDMEM) for the final 16 hr of culture. IL-2 was measured by [3H]thymidine incoporation of CTLL-2 cells (TIB-214, ATCC) or by ELISA. VSG-specific Th cell hybridomas Trypanosome infected B10 mice were euthanized

(17) Analysis of mitogenic activity of proteins after separation by gel electrophoresisCytotechnologyNovember 13, 2008Otmar Hohenwarter, Gorji Marzban, Elisabeth Jisa, Karola Vorauer-Uhl, Hermann Katingerwas prepared in our laboratory according to the method of Maciag et al. (1979). CTLL-2 cells (ATCC No TIB 214) were cultivated in suspension culture in medium RPMI 1640 plus 10% foetal calf serum, 110 mg l−1 sodiumpyruvate

(18) Degradation of cellular mRNA is a general early apoptosis‐induced eventThe FASEB JournalDecember 1, 2002M. Julieta Del Prete, Maria S. Robles, Ana Guío, Carlos Martínez‐A, Manuel Izquierdo, Jose A. Garcia‐Sanz(ATCC No. TIB208) is a mature murine (BALB/c) B cell line (30) (provided by Dr. Brás). CTLL-2 (ATCC No. TIB214) is a mouse cytotoxic T-cell clone requiring exogenous interleukin 2 (IL-2) for growth (31) (a gift

(19) Molecular Requirements for T Cell Recognition by a Major Histocompatibility Complex Class II–restricted T Cell Receptor: The Involvement of the Fourth Hypervariable Loop of the Vα DomainJ. Exp. MedFebruary 1, 1999Jayant Thatte, Ayub Qadri, Caius Radu, E. Sally Wardafter a single freeze-thaw, 80 μl aliquots were incubated with an IL-2–dependent cell line, CTLL-2 (TIB214; ATCC; 5 × 103 cells/well). Cells were pulsed 24 h later with 1 μCi of 3H/well for 16–18 h and thymidine

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CTLL-2 from ATCC

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