Anti-NRF1 antibodies are used in the immunodetection of the protein nuclear respiratory factor 1. In humans, the canonical protein has a reported length of 503 amino acid residues and a mass of 53.5 kDa. Its subcellular localization is in the nucleus. Alternative splicing is reported to yield 4 different isoforms for this protein. It is widely expressed across many tissue types. A member of a the NRF1/Ewg protein family, NRF1 is reported to be a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Post-translational modifications have been described, including phosphorylation. Synonyms for this target antigen include ALPHA-PAL and alpha palindromic-binding protein.