Excellent NRF1 Antibody

Nanomedicine
HMRI
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-NRF1 antibody [2F9] (ab55744)

Catalog Number:

ab55744

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: NRF1 Antibodies

I used this antibody with the aim to evaluate mitochondria biogenesis pathway in A549 and MRC5 cells. I diluted this antibody at a concentration 1:1000 in Non-Fat Dry Milk 5%. Predicted molecular weight was accurate and my Western Blots analysis result in a very specific and clean band. Highly recommended.

Experimental Design and Results Summary

Applications

Western Blot

Sample

A549, MRC5 cells

Primary Incubation

1:1000 overnight

Blocking Agent

5% NFDM in TBST 1x

Secondary Incubation

1:5000 2 hours

Tertiary Incubation

N/A

Detection

Western Blot

Results Summary

Diluted 1:1000 in 5% NFDM/TBST 1x, it gives very specific and high quality bands in WB.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specificity

The Bad

Nothing to complain.

The Bottom Line

Highly recommended

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