NDUFS3 Antibody

March 26, 2018

BlueskyReddit
Developmental and Molecular Biology
About Einstein College of Medicine
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology

Product Name:

NDUFS3 antibody

Catalog Number:

SC374282

Fairly easy to optimize based on instructions given with product. Clean band with little background using either whole cell lysate or mitochondrial extract.

Experimental Design and Results Summary

Applications

Western Blot

Sample

293T mitochondrial lysates and mouse smooth muscle cell mitochondrial lysates

Primary Incubation

1:500, overnight, 4 degrees

Blocking Agent

5% milk

Secondary Incubation

m-IgGκ BP-HRP, 1 hr, 24 degrees

Tertiary Incubation

None

Detection

ECL

Results Summary

Clean, single band at 30 kDa. At 1:500 dilution, ECL gave optimal signal after 1.5 minutes. Reproducible with 293T mitochondrial lysates and mouse smooth muscle cell mitochondrial lysates.

DOI or PMID #

27828948

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Ease of optimization

The Bad

None

The Bottom Line

Good, reliable antibody

Share your experience with other scientists. Write a Review! »

Join the discussion