Anti-SMAD5 antibodies are used in the immunodetection of the protein SMAD family member 5. In humans, the canonical protein has a reported length of 465 amino acid residues and a mass of 52.3 kDa. Its subcellular localization is in the nucleus and cytoplasm. It is ubiquitously expressed across many tissue types. A member of the Dwarfin/SMAD protein family, SMAD5 is known to be involved with the morphogenesis of anatomical structure and bone development. Post-translational modifications have been described, including protein cleavage and phosphorylation. Synonyms for this target antigen include JV5-1, MADH5, mothers against decapentaplegic homolog 5, MAD, mothers against decapentaplegic homolog 5, and DWFC. SMAD5 gene orthologs have been reported in the mouse, rat, bovine, zebrafish, chimpanzee and chicken species. Over 1200 citations in the literature describe the use of SMAD5 antibodies in research. Western Blot is a widely used application for these antibodies. Immunocytochemistry, Immunofluorescence, and Immunohistochemistry are also common applications.