Smad5 (D4G2) Rabbit mAb 12534S from Cell Signaling Technology

Product Specs
ItemSmad5 (D4G2) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number12534S
Quantity100 µl
ApplicationsW, IP, ChIP
ReactivityH, M, R, Mk
TargetSmad5
HostRabbit
NCBI Full Gene NameSMAD family member 5
NCBI Gene AliasesDWFC, JV5-1, MADH5
ClonalityMonoclonal
Research AreaDevelopmental Biology
CloneD4G2
Molecular Weight60

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Fstl1 determines luminal size of mouse trachea through negative regulation of BMP4 signalingiScienceAugust 21, 2026Yueyue Jin, Yue Zhang, Yingying Liu, Maolin Yao, Chenjie Zhang, Shuzi Li, Yibo Ding, Zhuan Niu, Yue Han, Yan Geng, et al.AB_354962. Human FSTL1 antibody,R&D,Cat#AF1694;RRID: AB_2106831. Smad5,Cell Signaling Technology,Cat#12534 S;RRID: N/A. Smad1/5/8,Immunoway,Cat#YT4325;RRID: N/A. p-Smad1/5/8,Cell Signaling Technology,Cat#13820;RRID:

(2) Endothelial epigenetic senescence driven microglial activation mediates cardio-retinal neuroinflammation in heart failureTheranosticsJuly 13, 2026Mengdan Wang, Shuo Zhang, Tursunjan Aziz, Shiyao Zhang, Xiao Wu, Gang Li, Yan WangSMAD2 (CST, 3103; 1:1000), SMAD2/3 (CST, 8685; 1:1000), p-SMAD1/5/9 (CST, 13820; 1:1000), SMAD5 (CST, 12534; 1:1000), EP300 (Invitrogen, MA1-16608; 1:1000; or Santa Cruz, sc-48343; 1:1000), Histone H3 (CST, 14269;

(3) ATG7 vs. ATG5: Distinct autophagy pathways shaping TGF-β signaling and endothelial functionPLoS OneApril 30, 2026Aman Singh, Bharatsinai Peddi, Kriti S. Bhatt, Hien C. Nguyen, Krishna K. Singh#8558), p21 (Cell Signaling # 2947S), SMAD3 (Cell Signalling, Cat #9523), SMAD5 (Cell Signalling, Cat #12534), LC3 (Proteintech, Cat #14600–1-AP) and GAPDH (Cell Signaling # 2118S). The proteins were then incubated

(4) Intrinsic mechanisms and microenvironmental cues fine-tune plasticity of esophageal progenitorsNature CommunicationsMarch 20, 2026Louison Descampe, Benjamin Dassy, Fadi Charara, Ligia Craciun, Laurine Verset, Sheleya Pirard, Quentin Verheye, Morgane Leprovost, Marie Isabelle Garcia, Alizée Vercauteren Drubbel, et al.Cell Signaling Technology, cat 5339), anti-Smad1 (rabbit monoclonal, Cell Signaling Technology, cat 12534), anti-Smad5 (rabbit monoclonal, Cell Signaling Technology, RRID:AB_2797946), anti-GAPDH (rabbit monoclonal,

(5) Involvement of c-Myc/WWP1/TRIM65 Axis in Renal FibrosisBiomoleculesMarch 2, 2026Sonia Mazumder, Cody Gifford, Jiaqi Tang, Fortis Gaba, Varsha Mondal, Roel Goldschmeding, Rohan Samarakoon, Paul J. HigginsSanta Cruz Biotechnology (Dallas, TX, USA), rabbit anti-p21 (1:1000; 2947), rabbit anti-SMAD5 (1:1000; 12534), rabbit anti-phospho-SMAD1/5 (1:1000; 9516), rabbit anti-snail (1:1000; 3879), rabbit anti-phospho

(6) OSMR coordinates a self-perpetuating circuit linking chemoresistance and neutrophil-driven immunosuppression in gastric cancerNeoplasiaFebruary 6, 2026Dan Wei, Jiang Chen, Feihu Bai, Donglin Li, Jie Liu, Hongjia Dou, Yan Lei, Yongzhen Zhang, Bo Zhang, Ying Pang, et al.#20326), TRIM2 (Proteintech, #20356-1-AP), GATA3 (CST, #5852), p-SMAD1/5 (CST, #9516), SMAD5 (CST, #12534), PD-L1 (CST, #13684), BATF (CST, #8638), IL-31 (abcam, #ab62579)), which were diluted in accordance

(7) POSTN+ fibroblast‑secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signalingOncol RepFebruary 2, 2026Chenyang Zhang, Si Chen, Chenghui Qian, Wanqi Lv, Qian Zhang, Yanjin Wang, Yuqiong Wu, Xue Liu(Ser463/465)/Smad5(Ser463/465)/Smad9(Ser465/467) (cat. no. 13820; 1:1,000; Cell Signaling Technology, Inc.), Smad5 (12534; 1:1,000; Cell Signaling Technology, Inc.), Smad9 (cat. no. abs131190; 1:1,000; Absin Bioscience) and

(8) Melatonin affects trophoblast epithelial-to-mesenchymal transition and oxidative damage resistance by modulating GDF15 expression to promote embryo implantationCommun BiolMarch 8, 2025Guang Yang, Guidong Yao, Huihui Wang, Ran Jiang, Junnan Fang, Jingyi Hu, Yue Kong, Haixia Jin, Wenyan Song, Zhaoting Wu, et al.Cell signaling technology, MA, USA, 1:1000), SMAD1 (6944, anti-rabbit, Cell signaling technology, 1:1000), SMAD5 (12534, anti-rabbit, Cell signaling technology, 1:1000), p-SMAD3 (9520, anti-rabbit, Cell signaling technology,

(9) A Tympanic Piezo‐Bioreactor Modulates Ion Channel‐Associated Mechanosignaling to Stabilize Phenotype and Promote Tenogenesis in Human Tendon‐Derived Cells.Advanced ScienceDecember 1, 2024Marc A. Fernandez‐Yague, Matteo Palma, Syed A. M. Tofail, Maeve Duffy, Leo R. Quinlan, Mathew J. Dalby, Abhay Pandit, Manus J. BiggsPathway, Cell Signaling, 5753S) relative to pan control (Total Smad1 and Smad5, Cell Signaling 6944S and 12534S). The activation of the WNT osteopecific was assessed by measuring active β‐catenin (clone 8E7 (Millipore,

(10) Targeting Tumor Heterogeneity by Breaking a Stem Cell and Epithelial Niche Interaction LoopAdvanced science (Weinheim, Baden-Wurttemberg, Germany)July 1, 2024Ma R, Feng D, Chen J, Zhou J, Xia K, Kong X, Hu G, Lu P.were visualized using anti‐SMAD1(Cell Signaling Technology (CST), #6944S, 1:1000), anti‐SMAD5(CST, #12534S, 1:1000), anti‐phospho‐SMAD1/5(CST, #9516S, 1:1000), anti‐BMP7(Santa Cruz Biotech, #sc‐53917, 1:1000),

(11) Control of neuronal excitation-inhibition balance by BMP-SMAD1 signallingNatureMay 1, 2024Okur Z, Schlauri N, Bitsikas V, Panopoulou M, Ortiz R, Schwaiger M, Karmakar K, Schreiner D, Scheiffele P.ChIP and 1:1,000 for western blot), H3K27ac (Abcam 4729, 1:1,000), rabbit anti-SMAD5 (Cell Signaling, 12534, 1:100 for ChIP and 1:1,000 for western blot), anti-phospho-SMAD1/5/9 (Cell Signaling 13820, 1:1,000),

(12) Self-promoted electroactive biomimetic mineralized scaffolds for bacteria-infected bone regenerationNature CommunicationsOctober 31, 2023Zixin Li, Danqing He, Bowen Guo, Zekun Wang, Huajie Yu, Yu Wang, Shanshan Jin, Min Yu, Lisha Zhu, Liyuan Chen, et al.Actin (1:1000, ZSGB-BIO, Cat#TA-09), p-Smad1/5/9 (1:1000, CST, Cat#13820), and Smad5 (1:1000, CST, Cat#12534), at a dilution of 1:1000 in 5% w/v skim milk overnight at 4 °C. Membranes were washed three times in

(13) GDF15 deficiency hinders human trophoblast invasion to mediate pregnancy loss through downregulating Smad1/5 phosphorylationiScienceOctober 20, 2023Yu-Ting Zeng, Wen-Fang Liu, Peng-Sheng Zheng, Shan Liphospho Smad3 (Ser423/425),Cell Signaling,#9520 Rabbit polyclonal antibody to Smad5,Cell Signaling,#12534 Rabbit polyclonal antibody to phospho Smad5(Ser463/465),Cell Signaling,#9516 Rabbit polyclonal antibody

(14) The TGF-β/UCHL5/Smad2 Axis Contributes to the Pathogenesis of Placenta AccretaInternational journal of molecular sciencesSeptember 5, 2023Hashimoto K, Miyagawa Y, Watanabe S, Takasaki K, Nishizawa M, Yatsuki K, Takahashi Y, Kamata H, Kihira C, Hiraike H, et al.anti-Phospho-Smad2 (18338), anti-Smad3 (9523), anti-Phospho-Smad3 (9520), anti-Smad4 (38454), anti-smad5 (12534), anti-Phospho-smad1/5 (9516), anti-Phospho-smad1/5/9 (13820), anti-ERK (9102), anti-Phospho-ERK (9101),

(15) 5-Azacytidine- and retinoic-acid-induced reprogramming of DCCs into dormancy suppresses metastasis via restored TGF-β-SMAD4 signalingCell reportsJune 27, 2023Singh DK, Carcamo S, Farias EF, Hasson D, Zheng W, Sun D, Huang X, Cheung J, Nobre AR, Kale N, et al.,Cell Signaling Technology,Cat#9743S; RRID: AB_2107780 Rabbit monoclonal anti-Smad5,Cell Signaling Technology,Cat#12534S; RRID: AB_2797946 Rabbit monoclonal anti-phospho-Smad1/5,Cell Signaling Technology,Cat#9516S; RRID:

(16) CHRDL1 Regulates Stemness in Glioma Stem-like CellsCellsDecember 3, 2022Berglar I, Hehlgans S, Wehle A, Roth C, Herold-Mende C, Rödel F, Kögel D, Linder B.Cambridge, UK); SMAD1 [(#6944, Cell Signaling Technology (CST), Danvers, MA, USA)] 1:1000; SMAD5 [(#12534, CST)] 1:1000; pSMAD1/5 [(#9516, CST)] 1:1000. The following secondary antibodies were used at a dilution

(17) ID1 and CEBPA coordinate epidermal progenitor cell differentiationDevelopment (Cambridge, England)November 15, 2022Kantzer CG, Yang W, Grommisch D, Vikhe Patil K, Mak KH, Shirokova V, Genander M.#9516, 1:1000). Smad1 (Cell Signaling Technology, #6944, 1:1000), Smad 5 (Cell Signaling Technology, #12534, 1:1000), Krt 10 (Covance, 90546, 1:1000). Plasmid information Lentiviral plasmids used for silencing

(18) BMP9-ID1 Signaling Activates HIF-1α and VEGFA Expression to Promote Tumor Angiogenesis in Hepatocellular CarcinomaInternational Journal of Molecular SciencesJanuary 27, 2022Han Chen et al.anti-phospho-Smad1/5 (Ser463/465) monoclonal #9576 (Cell Signaling Technology); anti-Smad5 monoclonal #12534 (Cell Signaling Technology); and anti-β-actin monoclonal, #4970 (Cell Signaling Technology). Immune

(19) LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP SignalingInternational journal of medical sciencesJanuary 1, 2022Mu R, Chen B, Bi B, Yu H, Liu J, Li J, He M, Rong L, Liu B, Liu K, et al.Cell Signaling Technology, MA, USA), p-Smad1/5 (#9516, Cell Signaling Technology, MA, USA), Smad5 (#12534, Cell Signaling Technology, MA, USA) and GAPDH (60004-1-Ig, Proteintech, IL, USA). Odonto/osteogenic

(20) Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces PreneoplasiaCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Puri P, Grimmett G, Faraj R, Gibson L, Gilbreath E, Yoder BK.,Cell Signaling,9145,Immunofluorescence,1:1000 P-SMAD1/5/9,Sigma,AB3848-I,Immunofluorescence,1:1000 P-SMAD1/5,Cell Signaling,9516,Western blotting,1:1000 SMAD5,Cell Signaling,12534,Western blotting,1:1000 SMA,Sigma,A5228,Immunofluorescence,1:2000 VIL1,Proteintech,16488-1-AP,Immunofluorescence,1:1000 VIM,Cell Signaling

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Smad5 (D4G2) Rabbit mAb

Smad5 (D4G2) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Smad5 (D4G2) Rabbit mAb from Cell Signaling Technology

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