A Good Phospho SMAD3 Antibody from Abcam

January 07, 2020

BlueskyReddit
Immunology
University of Pittsburgh
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-Smad3 (phospho S423 + S425) antibody

Catalog Number:

ab52903

Clone Number:

EP823Y

We used this antibody to detect phosphorylated SMAD3 in activated D10.G4.1 mouse cells. Cells were activated by CD3/CD28 beads in the presence of IL-2 and TGF-β.

Experimental Design and Results Summary

Applications

Western Blot

Sample

D10.G4.1

Primary Incubation

1:1000 in TBS-t, overnight at 4 C

Blocking Agent

5% BSA

Secondary Incubation

1:2000 anti-rabbit HRP linked for 1 hour at room temperature

Tertiary Incubation

None

Detection

Chemiluminescence

Results Summary

We found that this antibody was able to detect phosphorylated SMAD3 better than other options we had tried.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works well at detecting pSMAD3

The Bad

None

The Bottom Line

This product is effective at detecting phosphorylated SMAD3 by western blotting. Very specific and clean detection.

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