Anti-NPHS2 antibodies are used in the immunodetection of the protein NPHS2 stomatin family member, podocin. In humans, the canonical protein has a reported length of 383 amino acid residues and a mass of 42.2 kDa. Its subcellular localization is in the ER and cell membrane. Up to 2 different isoforms have been reported for this protein. It is notably expressed in the kidney. A member of the Band 7/mec-2 protein family, NPHS2 is reported to play a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
The NPHS2 marker can be used to identify Podocytes.* Synonyms for this target antigen include SRN1, podocin, NPHS2, podocin, nephrosis 2, idiopathic, steroid-resistant (podocin), and PDCN. NPHS2 gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. Over 290 citations in the literature describe the use of NPHS2 antibodies in research. Western Blot is a widely used application for these antibodies. In addition, Immunohistochemistry is also a common application.
*HuBMAP Human Reference Atlas v1.4