Anti-NPHS2 antibody [EPR13820] ab181143 from Abcam

Product Specs
ItemAnti-NPHS2 antibody [EPR13820]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab181143
Quantity10 UL, 40 UL, 100 UL
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Concentration0.994 mg/ml
Gene NameNPHS2
ReactivityHuman, Mouse, Rat
TargetNPHS2
ClonalityMonoclonal
NCBI Gene AliasesPDCN, SRN1
Antibody TypePrimary
HostRabbit
CloneEPR13820
IsotypeIgG
Conjugate/TagUnconjugated

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Triptolide protects against podocyte injury in diabetic nephropathy by activating the Nrf2/HO-1 pathway and inhibiting the NLRP3 inflammasome pathwayRenal FailureDecember 31, 2023Chenlei Lv, Tianyang Cheng, Bingbing Zhang, Ke Sun, Keda Luincubated with rabbit primary antibodies anti-Nephrin (1:1000, ab216341, Abcam), anti-Podocin (1:10000, ab181143), anti-Nrf2 (1:1000, ab92946), anti-heme oxygenase-1 (HO-1) (1:1000, ab68477), anti-NLRP3 (1:1000, ab270449),

(2) Anti-VEGFR2 F(ab')2 drug conjugate promotes renal accumulation and glomerular repair in diabetic nephropathyNat CommunDecember 13, 2023Di Liu, Yanling Song, Hui Chen, Yuchan You, Luwen Zhu, Jucong Zhang, Xinyi Xu, Jiahao Hu, Xiajie Huang, Xiaochuan Wu, et al.blocked in blocking buffer for 30 min at room temperature, incubated with anti-NPHS2 (1:400, Abcam, ab181143), anti-nitrotyrosine (3 μg/mL. GeneTex, GTX30730), or anti-F4/80 ((1:400, Cell Signaling, 70076s), or

(3) Protective effect of GLP-1 analog liraglutide on podocytes in mice with diabetic nephropathyEndocrine ConnectionsOctober 1, 2023Shaomin Shi, Xinghua Chen, Wen Yu, Xiaolan Ke, Tean Maperoxidase, blocking, corresponding primary antibodies nephrin (1:1500, ab216341, Abcam), NPHS2 (1:2000, ab181143, Abcam), GLP-1R (1:250, ab218532, Abcam), overnight at 4°C, HRP-labeled goat anti-rabbit secondary antibody

(4) Renal glomerular and tubular responses to glutaraldehyde- polymerized human hemoglobinFrontiers in MedicineJune 13, 2023Matthew C. Williams, Xiaoyuan Zhang, Jin Hyen Baek, Felice D’Agnilloab5076), N-cadherin (NCAD, Cell Signaling, 13116S), E-cadherin (ECAD, Abcam, ab219332), podocin (Abcam, ab181143), podocalyxin (Abcam, ab203079), and heme oxygenase-1 (HO-1, Enzo Life Sciences, ADI-SPA-894). Sections

(5) Vitamin D/vitamin D receptor/Atg16L1 axis maintains podocyte autophagy and survival in diabetic kidney diseaseRenal failureDecember 1, 2022Shi L, Xiao C, Zhang Y, Xia Y, Zha H, Zhu J, Song Z.(catalog no. Ab216341, Abcam, UK), beclin1 (catalog no. NB500-249 Novus, USA), podocin (catalog no. Ab181143 Abcam, UK), synaptopodin (catalog no. sc-515842 Santa Cruz, USA), desmin (catalog no. Ab8592 Abcam,

(6) HOXA11-OS participates in lupus nephritis by targeting miR-124-3p mediating Cyr61 to regulate podocyte autophagyMolecular MedicineDecember 1, 2022Xiuhong Pan, Shanshan Chen, Ruiwen Shen, Sen Liu, Yanwu Youanti-nephrin primary antibody (batch No.: ab216341), and rabbit anti-podocin primary antibody (batch No.: ab181143) were obtained from Abcam (Cambridge, UK). Mouse anti-LC3B primary antibody (batch No.: K0420) was purchased

(7) The nephroprotective action of Passiflora edulis in streptozotocin-induced diabetesScientific ReportsOctober 20, 2022Ony AraÃºjo Galdino, Iago de Souza Gomes, Renato Ferreira de Almeida JÃºnior, Maria Imaculada ConceiÃ§Ã£o Ferreira de Carvalho, Bento JoÃ£o Abreu, Marcela Abbott GalvÃ£o Ururahy, Barbara Cabral, Silvana Maria Zucolotto Langassner, Karla Simone Costa de Souza, Adriana Augusto de Rezendeantibodies: rabbit monoclonal nephrin (ab216341; Abcam Plc, Cambridge, UK), rabbit monoclonal podocin (ab181143; Abcam Plc), rabbit polyclonal LRP2 (megalin) (PA5-67900; Invitrogen), rabbit polyclonal GAPDH (glyceraldehyde-3-phosphate

(8) Activation of TRPC6 by AngⅡ Induces Podocyte Injury and Participates in Proteinuria of Nephrotic SyndromeFrontiers in PharmacologyAugust 3, 2022Ye Feng, Manman Li, Yunlai Wang, Mo Yang, Gaoxiang Shi, Dengke Yin, Zihua Xuan, Fan XuLtd, China. The primary antibodies against cleaved Caspase-3 (ab32351), TRPC6 (ab233413), and Podocin (ab181143) were purchased from Abcam Company, Britain. The secondary antibodies horseradish peroxidase (HRP)–conjugated

(9) DNA methyltransferase 1 knockdown reverses PTEN and VDR by mediating demethylation of promoter and protects against renal injuries in hepatitis B virus-associated glomerulonephritisCell & bioscienceJune 28, 2022Guan H, Zhu N, Tang G, Du Y, Wang L, Yuan W.anti-DNMT3b (#ab2851), anti-ZO1 (#ab216880), anti-VDR (#ab3508), anti-PTEN (#ab137337), anti-podocin (#ab181143), anti-fibronectin (#ab2413), anti-IL-6 (#ab9324, #ab208113)(Abcam, Cambridge, MA, UK), anti-p-NF-κB

(10) METTL3-mediated m(6)A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathyMolecular therapy : the journal of the American Society of Gene TherapyApril 6, 2022Jiang L, Liu X, Hu X, Gao L, Zeng H, Wang X, Huang Y, Zhu W, Wang J, Wen J, et al.antibody (Abcam, ab267377); anti-nephrin antibody (Abcam, ab216341); anti-podocin antibody (Abcam, ab181143); anti-MMP2 antibody (Proteintech, 10373-2-AP); anti-MT1-MMP antibody (Proteintech, 14552-1-AP); anti-IGF2BP2

(11) miR-199b-5p mediates adriamycin-induced podocyte apoptosis by inhibiting the expression of RGS10Experimental and therapeutic medicineDecember 1, 2021Qu G, He T, Dai A, Zhao Y, Guan D, Li S, Shi H, Gan W, Zhang A.(1:1,000; mouse; cat. no. sc-376522; Santa Cruz Biotechnology, Inc.), podocin (1:1,000; rabbit; cat. no. ab181143; Abcam), RGS10 (1:1,000; rabbit; cat. no. DF4414; Affinity Biosciences, Ltd.), Bax (1:1,000; rabbit;

(12) Inhibition of the Wnt/β‑catenin signaling pathway reduces autophagy levels in complement treated podocytesExperimental and Therapeutic MedicineMay 9, 2021Zhaocheng Dong et al.(1:1,000; cat. no. ab55811; Abcam), anti-podocin rabbit monoclonal antibody (mAb) (1:500; cat. no. ab181143; Abcam), anti-beclin-1 rabbit mAb (1:50; cat. no. ab217179; Abcam), anti-LC3A/B rabbit antibody (1:200;

(13) FOXA1 Suppresses SATB1 Transcription and Inactivates the Wnt/β-Catenin Pathway to Alleviate Diabetic Nephropathy in a Mouse ModelDiabetes, metabolic syndrome and obesity : targets and therapyJanuary 1, 2021Zhu H, Peng J, Li W.(OD) in each section was evaluated using the Image J. The primary sections used were Podocin (1:500, ab181143, Abcam Inc., Cambridge, MA, USA), FOXA1 (1:1000, ab170933, Abcam), SATB1 (1:100, ab109122, Abcam), β-catenin

(14) HGF protected against diabetic nephropathy via autophagy-lysosome pathway in podocyte by modulating PI3K/Akt-GSK3β-TFEB axisCellular signallingNovember 1, 2020Hou B, Li Y, Li X, Zhang C, Zhao Z, Chen Q, Zhang N, Li H.classification of glomerular diseases (1982 and 1995). Antibodies and reagents Antibodies against NPHS2/Podocin (ab181143), SQSTM1/p62 (ab109012), Akt (ab32505), GSK3β (ab32391), GAPDH (ab181602), rabbit IgG H&L (Alexa Fluor

(15) A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activationDiabetes, metabolic syndrome and obesity : targets and therapyJanuary 1, 2019Zhang C, Zhu X, Li L, Ma T, Shi M, Yang Y, Fan Q.wl02208, 1:200), phosphornuclear factor-κB p65 (p-NF-κB) (Wanleibio, wl02169, 1:100), podocin (Abcam, ab181143, 1:500), ﬁbronectin (Proteintech, 15613-1-AP, 1:200), α-SMA (Abcam, ab5694, 1:50), NLRP3 (Cell Signaling Technology

(16) Glucagon‑like peptide‑1 protects mouse podocytes against high glucose‑induced apoptosis, and suppresses reactive oxygen species production and proinflammatory cytokine secretion, through sirtuin 1 activation in vitroMolecular medicine reportsAugust 1, 2018Shi JX, Huang Q.following primary antibodies: Anti‑SIRT1 (ab28170; 1:1,000), anti‑nephrin (ab58968; 1:500), anti‑podocin (ab181143; 1:2,000), anti‑caspase‑3 (ab44976; 1:500), anti‑caspase‑9 (ab2013; 1:1,000) and anti‑GAPDH (5174; 1:2,000).

(17) Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5Kidney & blood pressure researchJanuary 1, 2018Yang X, Ding Y, Yang M, Yu L, Hu Y, Deng Y.1:1000, cat no. Ab66064, Abcam; anti-p53, 1:500, cat no. Ab26, Abcam; anti-Podocin, 1:500, cat no. Ab181143, Abcam; anti-Nestin, 1:500, cat no. ab6142, Abcam, or anti-β-actin, 1:100, cat no. SC-365062, Santa

(18) Berberine prevents the apoptosis of mouse podocytes induced by TRAF5 overexpression by suppressing NF-κB activationInternational Journal of Molecular MedicineNovember 7, 2017Feng Wu, Dong-Sheng Yao, Tian-Ying Lan, Chen Wang, Jian-Dong Gao, Li-Qun He, Di Huangby western blot analysis. **P<0.01 and ***P<0.001 vs. normal and negative control (NC). podocin (1:10,000; ab181143, Abcam) and anti‑GAPDH (1:1500; #5174; Cell Signaling Technology) antibodies; and mouse monoclonal anti-Bcl-2

(19) Advanced glycation end products induce the apoptosis of and inflammation in mouse podocytes through CXCL9-mediated JAK2/STAT3 pathway activationInternational Journal of Molecular MedicineOctober 1, 2017Jing Yu, Hao Wu, Zi-Yu Liu, Qi Zhu, Chang Shan, Ke-Qin Zhang1:1,000), anti-p-JAK2 (#3771; 1:1,000) (both from Cell Signaling Technology, Danvers, MA, USA), podocin (ab181143; 1:10,000; Abcam) and anti-GAPDH (#5174; 1:1,500; Cell Signaling Technology) antibodies, and mouse monoclonal

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Anti-NPHS2 antibody [EPR13820] from Abcam

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Anti-NPHS2 antibody [EPR13820] from Abcam

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