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Antibodies
Anti-NPHS2 antibody
Anti-NPHS2 antibody from Abcam
Be the first to write a review!
Citations:
(84)
Supplier Page
Supplier Page from
Abcam for
Anti-NPHS2 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-NPHS2 antibody
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab50339
Antibody Type
Primary
Host
Rabbit
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Isotype
IgG
Quantity
50 UL
Clonality
Polyclonal
Conjugate/Tag
Unconjugated
Format
ab50339 is affinity-purified using the immunizing peptide immobilized on agarose
Concentration
1.2 mg/ml
Gene Name
NPHS2
Reactivity
Mouse, Rat
Target
NPHS2
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (84)
(1)
Combination therapy with DHA and BMSCs suppressed podocyte injury and attenuated renal fibrosis by modulating the TGF-β1/Smad pathway in MN mice
Renal Failure
December 31, 2023
Yongzhang Li, Suzhi Chen, Jinchuan Tan, Yan Zhou, Meifang Ren, Qian Zhang, Meijiao Zhao, Guodong Yuan, Wenxi Zhang, Fengwen Yang
were incubated with primary antibodies against nephrin (
ab216341
,
1:1000
,
Abcam
, USA) and podocin (
ab50339
,
1:1000
;
Abcam
, USA) at 4 °C overnight. After the sections were incubated with secondary antibody at
(2)
A missense mutant of ocrl1 promotes apoptosis of tubular epithelial cells and disrupts endocytosis and the cell cycle of podocytes in Dent-2 Disease
Cell Commun Signal
December 5, 2023
Limin Huang, Yingying Zhang, Haidong Fu, Weizhong Gu, Jianhua Mao
tissues were incubated overnight at 4 °C with
anti-OCRL1
(#
8797
; CST, dilution
1:1000
), anti-podocin (#
ab50339
;
Abcam
, dilution
1:1000
), or anti-nephrin (#
AF3159
;
R&D Systems
, dilution
1:250
) antibodies. The following
(3)
tRF-003634 alleviates adriamycin-induced podocyte injury by reducing the stability of TLR4 mRNA
PLOS ONE
October 19, 2023
Xiaoqing Gao, Yunyang Qiao, Shanwen Li, Huimin Shi, Gaoting Qu, Jialing Ji, Weihua Gan, Aiqing Zhang
(
1:1000
,
2118
,
Cell Signaling
, USA), anti-nephrin (
1:1000
,
ab58968
,
Abcam
, UK), anti-podocin (
1:1000
,
ab50339
,
Abcam
, UK), anti-toll-like receptor 4 (TLR4,
1:1000
,
AF7017
,
Affinity
, USA), anti-
IL-6
(
1:1000
,
DF6087
,
(4)
Preparation of Human Kidney Progenitor Cultures and Their Differentiation into Podocytes
Bio Protoc
August 20, 2023
Maria Elena Melica, Maria Lucia Angelotti, Giulia Antonelli, Anna J. Peired, Carolina Conte, Letizia De Chiara, Benedetta Mazzinghi, Elena Lazzeri, Laura Lasagni, Paola Romagnani
μg/mL Sir-Actin,Spirochrome,SC001,1 μM
Nephrin
(
NPHS1
),
R&D system
,
AF4269
,4 μg/mL Podocin (NPHS2),
Abcam
,
ab50339
,30 μg/mL Secondary antibody,Secondary antibody,Secondary antibody,Secondary antibody Goat anti-mouse
(5)
Podocytes are likely the therapeutic target of IgA nephropathy with isolated hematuria: Evidence from repeat renal biopsy
Frontiers in Pharmacology
April 7, 2023
Mian-Na Luo, Yanqing Yin, Shangmei Li, Junfeng Hao, Cuiwei Yao, Yong-Zhi Xu, Hua-feng Liu, Lawei Yang
analysis for tissues was conducted as described previously (Liu et al., 2014). Rabbit
anti-NPHS2
(
Abcam
ab50339
) and
mouse
anti-4-HNE (
Abcam
ab48506
) antibodies were obtained. Alexa Fluor™ 488 donkey anti-rabbit
(6)
Mitofusin2 expression is associated with podocyte injury in IgA nephropathy
European journal of medical research
March 30, 2023
Tang X, Yuan Y, Liang X, Jiang X.
antibody (
1:100
; Cat No.
M6319
,
Sigma-Aldrich
), rabbit anti-
human
nephrin monoclonal antibody (
1:100
;
ab50339
,
Abcam
),
rat
anti-human collagen IV alpha 5 monoclonal antibody (
1:100
; C-452, Cosmo Bio), rabbit anti-
human
(7)
BaoShenTongLuo formula protects against podocyte injury by regulating AMPK-mediated mitochondrial biogenesis in diabetic kidney disease
Chinese medicine
March 26, 2023
Guo Y, Wang M, Liu Y, Pang Y, Tian L, Zhao J, Liu M, Shen C, Meng Y, Wang Y,
et al
.
for 30 min, followed by
incubation
with anti-nephrin (
1:2000
,
ab216341
,
Abcam
), anti-podocin (
1:1000
,
ab50339
,
Abcam
) and
anti-cleaved caspase-3
(
1:400
,
19677-1-AP
,
Proteintech
, Wuhan, China) antibodies overnight
(8)
Integrated analysis of RNA methylation regulators crosstalk and immune infiltration for predictive and personalized therapy of diabetic nephropathy
Human genomics
February 10, 2023
Li J, Liu D, Ren J, Li G, Zhao Z, Zhao H, Yan Q, Duan J, Liu Z.
were subjected to
immunoblot
analysis using standard methods with the following antibodies: NHPS2 (
ab50339
,
Abcam
), Synaptopodin (21064-1-AP),
METTL3
(
96391
s,
Cell Signaling
),
YTHDC1
(
77422
s,
Cell Signaling
),
(9)
Nicorandil protects podocytes via modulation of antioxidative capacity in acute puromycin aminonucleoside-induced nephrosis in rats
American Journal of Physiology-Renal Physiology
February 1, 2023
Masaki Yamanaka, Yoshifuru Tamura, Emiko Kuribayashi-Okuma, Shunya Uchida, Shigeru Shibata
serum complex, and then incubated at 4°C overnight with rabbit anti-podocin antibody (
1:2,000
dilution,
ab50339
,
Abcam
, Cambridge, MA) as the primary antibody. After
incubation
with the secondary antibody for 1 h
(10)
ZEB2 controls kidney stromal progenitor differentiation and inhibits abnormal myofibroblast expansion and kidney fibrosis
JCI insight
January 10, 2023
Kumar S, Fan X, Rasouly HM, Sharma R, Salant DJ, Lu W.
(
13820
,
Cell Signaling Technology
;
1:100
dilution), AXIN2 (
ab32197
,
Abcam
;
1:100
dilution), and podocin (
ab50339
,
Abcam
;
1:500
dilution). A list of cell-specific markers used in this study is shown in Supplemental
(11)
Identification of ARMH4 and WIPF3 as human podocyte proteins with potential roles in immunomodulation and cytoskeletal dynamics
PloS one
January 1, 2023
De Luca F, Kha M, Swärd K, Johansson ME.
HPA001580
at
1:200
;
anti-WIPF3
:
Atlas Antibodies
, Cat#
HPA041145
at
1:200
;
anti-NPHS2
:
Abcam
, Cat#
ab50339
at
1:200
or
Sigma
, Cat#
SAB4200810
at
1:500
; anti-
WT1
:
Abcam
, Cat#
ab212951
at
1:500
) diluted in PBS
(12)
A novel nanoluciferase transgenic reporter to measure proteinuria in zebrafish
Kidney International
October 1, 2022
Richard W. Naylor, Emmanuel Lemarie, Anthony Jackson-Crawford, J. Bernard Davenport, Aleksandr Mironov, Martin Lowe, Rachel Lennon
CM1950 cryostat and processed for antibody
staining
. The primary antibodies used were NPHS2 (
Abcam
#
ab50339
;
1:250
) and pan collagen IV (
Abcam
#
ab6586
;
1:250
). Images were collected on a Leica TCS SP8 AOBS inverted
(13)
Integrated genomic, transcriptomic and metabolomic analysis reveals MDH2 mutation-induced metabolic disorder in recurrent focal segmental glomerulosclerosis
Frontiers in Immunology
September 8, 2022
Qixia Shen et al.
temperature prior to
incubation
with synaptopodin antibody (
1:100
, sc-21537, Santa),
NPHS2 antibody
(
1:100
,
ab50339
,
Abcam
),
Megsin
(
1:100
,
bs-0815R
,
Bioss
) overnight in a humidified chamber at 4°C. Slides were incubated
(14)
CD36 aggravates podocyte injury by activating NLRP3 inflammasome and inhibiting autophagy in lupus nephritis
Cell death & disease
August 23, 2022
Lv F, He Y, Xu H, Li Y, Han L, Yan L, Lang H, Zhao Y, Zhao Z, Qi Y.
Abcam), rabbit anti-MAP1LC3B (ab4839, Abcam4), rabbit anti-p62 (
ab91526
,
Abcam
), rabbit
anti-NPHS2
(
ab50339
,
Abcam
), rabbit anti-nephrin (
ab58968
,
Abcam
), and rabbit anti-
beta-Actin
(
20536-1-AP
,
Proteintech
).
(15)
Adult Mouse Kidney Stem Cells Orchestrate the De Novo Assembly of a Nephron via Sirt2-Modulated Canonical Wnt/β-Catenin Signaling
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
May 1, 2022
Han X, Sun Z.
(
1:100
,
StressMarq
SPC‐402
), rabbit anti‐CD31 (
1:200
,
Abcam
ab28364
), rabbit anti‐podocin (
1:200
,
Abcam
ab50339
), rabbit anti‐
Six2
(
1:200
,
Proteintech
#
11562‐1‐AP
),
goat
anti‐GATA‐3
(
1:200
, R&D AF2605‐SP), mouse
(16)
A Biomimetic Electrospun Membrane Supports the Differentiation and Maturation of Kidney Epithelium from Human Stem Cells
Bioengineering (Basel)
April 26, 2022
Xingrui Mou, Jessica Shah, Rohan Bhattacharya, Titilola D. Kalejaiye, Bowen Sun, Po-Chun Hsu, Samira Musah
primary antibodies used included nephrin (ARP, Waltham, MA, USA; GP-N2), podocin (
Abcam
, Boston, MA, USA;
ab50339
), and
Pax2
(
Invitrogen
, Waltham, MA, USA;
71-6000
). Following overnight
incubation
, samples were rinsed
(17)
SARS-CoV-2 Employ BSG/CD147 and ACE2 Receptors to Directly Infect Human Induced Pluripotent Stem Cell-Derived Kidney Podocytes
Frontiers in Cell and Developmental Biology
April 20, 2022
Titilola D. Kalejaiye, Rohan Bhattacharya, Morgan A. Burt, Tatianna Travieso, Arinze E. Okafor, Xingrui Mou, Maria Blasi, Samira Musah
Invitrogen
,
D1306
). The primary antibodies used were anti-
Nephrin
(
Progen
,
GP-N2
); anti-Podocin (
Abcam
,
ab50339
);
anti-SARS
-CoV2 spike (
ProSci
,
3525
); anti-SARS-CoV-2 N protein (
Sinobiological
,
40143-R019
); anti-GFP
(18)
Empagliflozin Ameliorates Preeclampsia and Reduces Postpartum Susceptibility to Adriamycin in a Mouse Model Induced by Angiotensin Receptor Agonistic Autoantibodies
Frontiers in Pharmacology
March 23, 2022
Ruonan Zhai, Yuan Liu, Jiahao Tong, Ying Yu, Lin Yang, Yong Gu, Jianying Niu
Pharmaceuticals Co. Ltd. (Merck Sharp & Dohme, Australia).
Anti-synaptopodin
(ab259976),
anti-NPHS2
(
ab50339
),
anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172
) (
ab133448
) and
anti-SOD2/MnSOD
(
ab68155
)
(19)
miR-188-3p abolishes germacrone-mediated podocyte protection in a mouse model of diabetic nephropathy in type I diabetes through triggering mitochondrial injury
Bioengineered
January 5, 2022
Yunguang Wang, Fangfang Feng, Wenfang He, Lifang Sun, Qiang He, Juan Jin
blocked in 5% BSA at 25°C for 1 h, and subsequently incubated with primary antibodies against podocin (
ab50339
,
Abcam
, Cambridge, UK,
1:200
) and nephrin (ABIN2177857, antibodies-online.com, Aachen, Germany,
1:200
),
(20)
GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening
Communications biology
December 2, 2021
Tuffin J, Chesor M, Kuzmuk V, Johnson T, Satchell SC, Welsh GI, Saleem MA.
respectively) (
1:100
), Laminin α5 (
Abcam
ab77175
) (
1:200
), fibronectin (
Abcam
ab2413
) (
1:50
), podocin (
Abcam
ab50339
) (0.2 µg/ml), nephrin (
Origene
BP5030
)(
1:50
),
PECAM-1
(
Cell Signalling
#
3528
)(
1:800
), podocalyxin (
ThermoFisher
Page
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Anti-NPHS2 antibody from Abcam
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