Anti-regulatory factor X5 antibodies are used for the immunodetection of the protein encoded by the RFX5 gene. In humans, the canonical protein has a reported length of 616 amino acid residues and a mass of 65.3 kDa. Its subcellular localization is in the nucleus. Alternative splicing is reported to yield 2 different isoforms for this protein. It is ubiquitously expressed across many tissue types. A member of the RFX protein family, it is known to be involved with the regulation of transcription. Post-translational modifications have been described, including phosphorylation. Other names for this target antigen include DNA-binding protein RFX5 and regulatory factor X, 5 (influences HLA class II expression). Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of regulatory factor X5 antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. In addition, ELISA is also a common application.