Immunoprecipitation Kits and Reagents

Immunoprecipitation kits are available from various suppliers for rapid and streamlined collection of target antigens or proteins. Immunoprecipitation (IP) is a form of affinity purification in which targets compounds are purified, or “precipitated” out of a complex solution via specific antibody binding. Once the target has bound to the antibody, there are two ways to collect the antibody-target complex. With pre-immobilized antibodies, the immunoglobulin is covalently linked to a solid support, such as agarose resin or magnetic particles. Alternatively, antibodies could be initially uncoupled, but collected later via immobilized antibody-binding proteins, protein A or protein G. Compared with other modes of protein purification, immunoprecipitation is typically intended for the small-scale collection of antigens from lysates or fluids for downstream analysis, such as assays or blotting.