Antibody Purification involves selective enrichment or specific isolation of antibodies from serum, ascites fluid, or cell culture supernatant. Purification is required to obtain antibody probes that can be used effectively in multiple types of detection methods. There are a variety of purification methods, ranging from non-specific to highly specific. The methods are classified under physiochemical fractionation, class-specific affinity, or antigen- specific affinity, in increasing order of specificity. When choosing the appropriate purification method keep in mind both the antibody of interest as well as the future downstream analysis.
Both mass spec & non-mass spec methods are expanding the field
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Overview of principles and biochemically pertinent considerations
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The FLUOstar Omega is a multi-mode microplate reader featuring BMG LABTECH's proprietary Tandem Technology. It uses an ultra-fast CCD-based spectrometer for UV/Vis absorbance and ultra-sensitive filters for detection in other modes. Unique to BMG ...
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Discover the beauty of standardized and automated flow cytometry
The MACSQuant® Analyzer 16 is an easy-to-use instrument designed for scalable and reliable flow cytometry assays. This includes features such as:
Full workflow automation: sample ...
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The Abcam PEG Virus Precipitation Kit is used to concentrate lentivirus from HEK293T supernatant, and it has been a great solution for labs that don't want to tie up an ultracentrifuge.
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The Superdex 200 Increase is a column made from agarose-dextran that enables size-dependent separation of proteins. It effectively separates proteins based on their size and offers excellent resolution specifically in the 100-300 kDa range. This ...
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