Antibody Purification

Antibody Purification Antibody Purification involves selective enrichment or specific isolation of antibodies from serum, ascites fluid, or cell culture supernatant. Purification is required to obtain antibody probes that can be used effectively in multiple types of detection methods. There are a variety of purification methods, ranging from non-specific to highly specific. The methods are classified under physiochemical fractionation, class-specific affinity, or antigen- specific affinity, in increasing order of specificity. When choosing the appropriate purification method keep in mind both the antibody of interest as well as the future downstream analysis.
  • Amicon Filters for Fast Protein Concentration

    I study gene signaling pathways and routinely concentrate purified proteins and protein samples. The Amicon Ultra provides a quick and convenient way to concentrate samples using a 30 kDa molecular-weight cutoff membrane. read more
  • Reliable Glutathione Agarose

    I regularly express and purify GST-tagged proteins for biochemical studies. This glutathione agarose resin provides a straightforward affinity purification method for GST-tagged proteins. read more