Cell and Protein Fractionation Kits

Cellular fractionation involves the gradual separation of membrane, nuclear, cytoplasmic and organelle components from cultured cells or tissue. Traditionally, these subcellular fractions can be laborious to separate due to their low concentration and propensity towards subcellular contamination. However, a variety of ready-to-use kits are now available, containing the necessary reagents to quickly and reproducibly enrich for the fractions of interest. Such tools can prove useful for downstream applications such as Western blots, 2D gel electrophoresis, enzyme activity assays, as well as in proteomic and genomic analyses. Consider the requirements of the intended application to determine the chemical compatibilities of the included reagents.