Lysosome Isolation Kit ab234047 from Abcam

Product Specs
ItemLysosome Isolation Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab234047
Quantity50 Test
Sample TypeTissue, Adherent cells, Suspension cells

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) DNA damage in macrophages drives immune autoreactivity via nuclear antigen presentationNature AgingJanuary 15, 2026George Niotis, Ermioni S. Arvanitaki, Emmanouil Theodorakis, Adrian Schmalen, Thomas Juretschke, Orestis Argyros, Konstantinos C. Tsolis, George Bertsias, Elias Drakos, Petra Beli, et al.from four mice were collected for the isolation of lysosomes using the Lysosome Isolation Kit (Abcam, ab234047). Lysosomes were then lysed using RIPA, and their protein content was detected through immunoblotting

(2) Bmal1 is involved in the regulation of macrophage cholesterol homeostasisJCI InsightNovember 10, 2025Xiaoyue Pan, John O’Hare, Cyrus Mowdawalla, Samantha Mota, Nan Wang, M. Mahmood Hussain(cytosol). In most experiments, the lysosome fraction was purified with a lysosome isolation kit (Abcam, Ab234047). Measurement of cholesterol accumulation and egress in lysosomes. To study cholesterol accretion,

(3) Activation of lysosomal iron triggers ferroptosis in cancerNatureJune 1, 2025Cañeque T, Baron L, Müller S, Carmona A, Colombeau L, Versini A, Solier S, Gaillet C, Sindikubwabo F, Sampaio JL, et al.(10 µM) for 1 h, and lysosome-enriched fractions were isolated using a Lysosome Isolation kit (Abcam, ab234047) according to the manufacturer’s protocol. In brief, 2 × 107 cells were washed and centrifuged at 600g

(4) ATP6AP1 promotes cell proliferation and tamoxifen resistance in luminal breast cancer by inducing autophagyCell Death DisMarch 25, 2025Zhengwei Yan, Aidi Huang, Dongwen Ma, Chenao Hong, Shengmiao Zhang, Luling He, Hai Rao, Shiwen Luo(MedChemExpress, HY-100558), LysoSensorTM Green DND-189 (YEASEN, 40767ES50), Lysosome Isolation Kit (Abcam, ab234047), Protein A-agarose beads (Roche, 11134515001) and Protein G-agarose beads (Roche, 11243233001), The

(5) Regulation of mammalian cellular metabolism by endogenous cyanide productionNat MetabMarch 3, 2025Karim Zuhra, Maria Petrosino, Lucia Janickova, Jovan Petric, Kelly Ascenção, Thibaut Vignane, Moustafa Khalaf, Thilo M. Philipp, Stella Ravani, Abhishek Anand, et al.glass-Teflon Potter–Elvehjem homogenizer and homogenized (12 strokes) in 1 ml of lysosome isolation buffer (ab234047, Abcam), supplemented with phosphatase/protease inhibitor cocktail (Halt, 1861281) on ice. After homogenization,

(6) Sodium pump subunit NKAα1 protects against diabetic endothelial dysfunction by inhibiting ferroptosis through the autophagy-lysosome degradation of ACSL4Clinical and translational medicineFebruary 1, 2025Zhu XX, Su JB, Wang FM, Chai XY, Chen G, Xu AJ, Meng XY, Qiu HB, Sun QY, Wang Y, et al.fractions from tissues and cells were enriched and purified using the Lysosome Isolation Kit (Abcam, ab234047) by differential centrifugation followed by density gradient centrifugation. 46 , 47 Briefly, samples

(7) Discovery of a new class of cell-penetrating peptides by novel phage display platformScientific ReportsJune 11, 2024Jinsha Liu, John Heddleston, Douglas Raymond Perkins, Jack Jia Hua Chen, Ahmadreza Ghanbarpour, Bill William Smith, Rebecca Miles, Eitaro Aihara, Sepideh Afsharextraction. Lysosomal isolation from different cell types were optimized based on Abcam kit (Cat. No. ab234047) for homogenization step and increased isolation scale. The starting cell number for one lysosomal isolation

(8) Spatiotemporally mapping temperature dynamics of lysosomes and mitochondria using cascade organelle-targeting upconversion nanoparticlesProceedings of the National Academy of SciencesNovember 8, 2022Xiangjun Di, Dejiang Wang, Qian Peter Su, Yongtao Liu, Jiayan Liao, Mahnaz Maddahfar, Jiajia Zhou, Dayong Jinwater was generated by Milli-Q IQ Water Purification System (Millipore). The Lysosome Isolation Kit (ab234047) and the Lysosomal Intracellular Activity Assay Kit (ab234622) were purchased from Abcam. Synthesis

(9) DCN released from ferroptotic cells ignites AGER-dependent immune responsesAutophagySeptember 1, 2022Liu J, Zhu S, Zeng L, Li J, Klionsky DJ, Kroemer G, Jiang J, Tang D, Kang R.Centrifugal Filter Unit with Ultracel-10 membrane (Millipore, MRCPRT010). The lysosome isolation kit (Abcam, ab234047) was used to isolate lysosomal fractions from cultured cells by differential centrifugation followed

(10) Mannose ameliorates experimental colitis by protecting intestinal barrier integrityNature communicationsAugust 16, 2022Dong L, Xie J, Wang Y, Jiang H, Chen K, Li D, Wang J, Liu Y, He J, Zhou J, et al.processing. Lysosome and mitochondria isolation Lysosomes were isolated by a lysosome isolation kit (Abcam, ab234047) according to the manufacturer’s protocol. In brief, the cells were isolated in ice-cold lysosome isolation

(11) Endocytosed HIV-1 Envelope Glycoprotein Traffics to Rab14(+) Late Endosomes and Lysosomes to Regulate Surface Levels in T-Cell LinesJournal of virologyJuly 27, 2022Hoffman HK, Aguilar RS, Clark AR, Groves NS, Pezeshkian N, Bruns MM, van Engelenburg SB.membranes). Lysosomal enrichment and isolation were performed using reagents from the Lysosomal isolation kit (ab234047; Abcam). Cells were resuspended in lysosome isolation buffer containing Protease Inhibitor Cocktail

(12) Protein-lipid interaction at low pH induces oligomerization of the MakA cytotoxin from Vibrio choleraeeLifeFebruary 8, 2022Aftab Nadeem, Alexandra Berg, Hudson Pace, Athar Alam, Eric Toh, JÃ¶rgen Ã…dÃ©n, Nikola Zlatkov, Si Lhyam Myint, Karina Persson, Gerhard GrÃ¶bner, et al.lysosomes were purified from vehicle- or MakA-treated HCT8 cells using the Lysosome Isolation Kit (ab234047, Abcam), according to the manufacturer’s instructions. For the lysosome pull-down assay, intact lysosomes

(13) Inhibitory role of TRIP-Br1 oncoprotein in anticancer drug-mediated programmed cell death via mitophagy activationInternational journal of biological sciencesJanuary 1, 2022Jung S, Myagmarjav D, Jo T, Lee S, Han S, Quynh NTN, Anh NH, Vu SH, Choi Y, Lee MS.analysis. Preparation of lysosomal fractionations Lysosomes were isolated emplyoing a lysosome isolation kit (Abcam, #ab234047) following the manufacturer's protocol. Briefly, cells were collected into 15 mL conical tubes and isolated

(14) Synthesis of an Hsp70 inhibitor and its assessment of lysosomal membrane permeabilizationSTAR protocolsMarch 19, 2021Park SH, Park S, Shin I.assays,Critical commercial assays,Critical commercial assays ,, Lysosome isolation kit,Abcam,Cat#ab234047 ,, Experimental models: cell lines,Experimental models: cell lines,Experimental models: cell lines ,, HeLa

(15) Neurodevelopmental defects and neurodegenerative phenotypes in human brain organoids carrying Parkinson’s disease-linked DNAJC6 mutationsScience AdvancesFebruary 19, 2021Noviana Wulansari et al.Lysosomal GBA activity was measured from the lysosome fractions obtained by using a kit from Abcam (ab234047) according to the manufacturer’s instruction. FM 1-43 styryl dye uptake Cells were washed and incubated

(16) TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stressCell death & diseaseJanuary 7, 2021Guo S, Chen Y, Yang Y, Zhang X, Ma L, Xue X, Qiao Y, Wang J.Beyotime). Lysosome and proteasome isolation Lysosomes were isolated by a lysosome isolation kit (ab234047, Abcam) according to the manufacturer’s instructions. The presence of the lysosomes and the purity of

(17) Extracellular SQSTM1 mediates bacterial septic death in mice through insulin receptor signallingNature microbiologyDecember 1, 2020Zhou B, Liu J, Zeng L, Zhu S, Wang H, Billiar TR, Kroemer G, Klionsky DJ, Zeh HJ, Jiang J, et al.Centrifugal Filter Unit with Ultracel-10 membrane (MRCPRT010, Millipore). The lysosome isolation kit (ab234047, Abcam) was used to isolate lysosomal fractions from cultured cells by differential centrifugation followed

(18) Clockophagy is a novel selective autophagy process favoring ferroptosisScience AdvancesJuly 24, 2019Minghua Yang, Pan Chen, Jiao Liu, Shan Zhu, Guido Kroemer, Daniel J. Klionsky, Michael T. Lotze, Herbert J. Zeh, Rui Kang, Daolin Tangused to assay cell death. Lysosome isolation Lysosomes were isolated using a lysosome isolation kit (ab234047, Abcam) according to the manufacturer’s protocol. Briefly, cells were isolated in ice-cold lysosome

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Lysosome Isolation Kit from Abcam

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Lysosome Isolation Kit from Abcam

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