Great Kit for RNA Extraction

Pediatric Neurology, CU Anschutz
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Questions/Comments

Company:

Qiagen

Product Name:

RNeasy Plus Kit

Catalog Number:

74134

I work on using stem cell models to understand and treat rare pediatric diseases. This kit has been utilized on various cell types and patient lines to extract RNA for uses including RNA-sequencing and qPCR.

Experimental Design and Results Summary

Application

RNA Extraction

Starting Material

Human-derived differentiated oligodendrocytes

Results Summary

The kit does a great job in extracting DNA from a variety of samples with the gDNA columns eliminating additional DNAse steps. Extracted RNA has been measured on a NanoDrop machine and demonstrated good quantity and quality. Image attached demonstrates quantity and quality of some extracted samples.

DOI or PMID #

https://doi.org/10.1186/s12967-026-08708-8

Image Gallery

Summary

The Good

Easy protocol with built-in gDNA eliminator

The Bad

More expensive than regular RNEasy kit

The Bottom Line

Overall, a more expensive but higher quality RNA extraction kit with an easy protocol and good efficiency.

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