Santa Cruz Technologies
Vinculin Antibody
sc-73614
7F9
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We study cutaneous melanoma and use this vinculin antibody as a western blot loading control for both human and mouse cell lines.
Western Blot
Human and murine cutaneous melanoma
1:1000, o/n at 4C
5% BSA
1:4000, 1 hour at room temperature
N/A
Chemiluminescence on Biorad ChemiDoc
Works well as a protein loading control for western blot in multiple systems. Showing here in 1014 murine melanoma cells. Sometimes produces off target/non-specific bands, but overall results are consistent.
Reliable
Some off target bands
Great for use as Western blot loading control