Cell Signaling Technologies
HSP90 Rabbit Monoclonal Antibody
4877
C45G5
Browse Similar Products: HSP90 Rabbit Monoclonal Antibody Products
We study cutaneous melanoma (human and murine cell lines) and use this HSP90 antibody as a loading control for Western blots every week. Very reliable both in vitro and in vivo.
Western Blot
Human and murine cutaneous melanoma
1:1000
5% BSA
1:4000, 1 hour at room temperature
N/A
Chemiluminescence on Biorad ChemiDoc
Here, I used the HSP90 antibody to validate protein levels in lysates collected from murine melanoma tumors. Works wonderfully as a loading control. My only note is that after multiple uses as a primary antibody, images take longer to develop. I have not had this problem with other loading control antibodies.
Very reliable, consistent signal
Loses potency over time
Highly recommend as loading control antibody for Western blots