Excellent Na,K-ATPase Antibody

September 29, 2026

BlueskyReddit
Biochemistry
Stanford University
Senior Research Scientist

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Company:

Cell Signaling

Product Name:

Na,K-ATPase Antibody

Catalog Number:

3010

Info:View Product Specs

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WNT signaling is essential for embryonic development and adult tissue homeostasis, and its aberrant activation drives several cancers, most notably colorectal cancer. My lab studies the molecular mechanisms of oncogenic WNT signal transduction, with the goal of identifying therapeutic vulnerabilities in colorectal cancer. Stabilized β-catenin acts in the nucleus with TCF/LEF transcription factors, so we use subcellular fractionation to follow pathway components across compartments during WNT stimulation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293T sub-cellular fractions

Primary Incubation

1:2000

Blocking Agent

5% milk in TBS-T (0.1% Tween-20)

Secondary Incubation

HRP-conjugated Donkey anti-Rabbit IgG

Tertiary Incubation

None

Detection

ECL regular

Results Summary

I use this antibody as a plasma membrane marker to assess fractionation quality. Membrane, cytosolic, and nuclear fractions were prepared from WNT3A-treated 293T cells by hypotonic swelling, needle shearing, and differential centrifugation, with the membrane fraction pelleted at 100,000 × g and washed. Samples were resolved on a 3–8% Tris-acetate gel, transferred to nitrocellulose, blocked in 5% milk/TBS-T, and probed with the antibody at 1:2000 overnight at 4 °C, followed by HRP-conjugated donkey anti-rabbit IgG (1:10,000) and ECL regular substrate. The antibody detected a strong band at approximately 100–110 kDa in every membrane fraction lane, with no detectable signal in any cytosolic or nuclear lane.

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Summary

The Good

As a compartment marker, this antibody does exactly what is needed: an unambiguous, high-intensity signal in the membrane fraction and complete absence from the other two fractions across eighteen lanes. That clean separation is what makes it possible to interpret the localization of other proteins on parallel blots with confidence. It works at 1:2000, so a vial goes a long way, and it performed well in a milk-based blocking system.

The Bad

None

The Bottom Line

Cell Signaling's Na,K-ATPase antibody is an excellent plasma membrane marker for sub-cellular fractionation studies. It gave a strong, membrane-exclusive signal with no cross-fraction background. I recommend it without hesitation.

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