Sensitive DVL3 Antibody for Western Blot

Results Summary

I used this antibody to blot whole-cell lysates from a panel of nine HEK293T lines carrying base-edited AXIN1 variants, together with non-targeting controls, all treated with WNT3A-conditioned medium and R-spondin 3. Lysates were prepared in a Triton X-100 buffer, and 50 µg per lane was resolved on a 3–8% Tris-acetate gel and transferred to nitrocellulose. Membranes were blocked in 5% milk/TBS-T, probed with the Dvl3 antibody at 1:1000 overnight at 4 °C, then with HRP-conjugated donkey anti-rabbit IgG (1:10,000) and detected with standard ECL. The antibody gave a single strong band at approximately 80 kDa, within the expected range for DVL3, with consistent signal across all nine lysates and no additional bands on the blot. Note that we did not include a DVL3 knockout or knockdown control in this experiment. Because DVL1, DVL2, and DVL3 are closely related paralogs of similar molecular weight, a DVL3-null sample would have been the best way to confirm that the detected band is DVL3 rather than a cross-reacting paralog. Readers who need definitive specificity should include such a control.