Excellent LAMP2 Antibody for Immunofluorescence

Department of Pathology
Stanford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech

Product Name:

CD107b / LAMP2 antibody

Catalog Number:

65053-1-Ig

Clone Number:

H4B4

Info:Supplier Page

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I study gene signaling pathways and lysosomal biology, and this antibody has been an excellent marker for visualizing lysosomes in cultured cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PFA-fixed human cell lines

Primary Incubation

1:200, 1 hour, Room temperature

Blocking Agent

Donkey serum and BSA

Secondary Incubation

1:500, 1 hour, Room temperature

Tertiary Incubation

NA

Detection

Confocal microscopy

Results Summary

The antibody produced robust, specific lysosomal staining, allowing reliable visualization of lysosome morphology and for co-localization studies in cells.

Additional Notes

Does not require aliquoting for storage. One vial lasts a long time, making it very cost-effective.

Image Gallery

Summary

The Good

Clean IF staining, easy to optimize, and excellent value for the price.

The Bad

None

The Bottom Line

A highly reliable and affordable lysosomal marker antibody.

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