Reliable and Easy Protein Quantification BCA Assay Kit

Stanford University
Department of Pathology
Postdoctoral Researcher

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Company:

Thermo Fisher Scientific

Product Name:

Pierce™ BCA Protein Assay Kit

Catalog Number:

23227

I study gene signaling pathways and routinely quantify protein samples before downstream experiments. This BCA assay is reliable, straightforward, and compatible with many common protein extraction buffers.

Experimental Design and Results Summary

Application

Protein concentration measurement

Starting Material

Cell and protein lysates

Tips

The assay time can be shortened by incubating at 50 degree C for 10 min.

Results Summary

Provides consistent protein concentration measurements, allowing me to normalize samples for Western blot, IP, and other protein-based assays. The kit has a broad working range of 20–2,000 µg/mL and can be used in microplate formats.

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Summary

The Good

Reliable, easy to use, broad working range, and compatible with many detergents.

The Bad

None

The Bottom Line

A dependable, routine protein quantification assay that can be used for normalizing protein samples.

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