Excellent CellTrace Violet Dye for Cell Tracking, Coculture Identification, and Proliferation Analysis

September 13, 2026

BlueskyReddit
School of Medicine
Lambe Institute for Translational Research
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

CellTrace™ Violet Cell Proliferation Kit, for flow cytometry

Catalog Number:

C34557

Labelling adherent MDA-MB-231 breast cancer cells with CellTrace Violet for cell identification and tracking in NK cell–tumour coculture assays.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

MDA-MB-231 breast cancer cell line

Primary Incubation

Add 0.1 µM CTV working solution at a maximum density of 1 × 10^6 cells/ml. Incubate for 20 minutes at 37 °C, protected from light. Quench the reaction by adding 500 µL of complete DMEM medium and remove the solution from the wells.

Blocking Agent

DMEM + 10% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Spectral flow cytometer Cytek NL2000

Results Summary

The CellTrace Violet dye provided very clear and consistent labelling of adherent MDA-MB-231 cells, with strong fluorescence achieved at a concentration well below the manufacturer’s recommended concentration.

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Summary

The Good

Provides very bright and clear staining of adherent MDA-MB-231 cells, with excellent fluorescence even at concentrations well below the manufacturer’s recommendation

The Bad

None

The Bottom Line

An excellent choice for labelling adherent MDA-MB-231 cells, providing bright and consistent fluorescence at very low concentrations, making it particularly useful for cell identification in coculture assays.

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