ThermoScientific
B-PER™ Complete Bacterial Protein Extraction Reagent
89821
Info:Supplier Page
I study gene signaling pathways and use bacterial expression systems for protein production. B-PER provides a simple and fast way to lyse bacterial pellets and consistently improves protein recovery.
Bacterial cell lysis for protein extraction
Bacterial cell pellets
Depending on the application, protease inhibitors, reducing agents, and chelating agents may be added to the reagent.
I used the reagent at 4ml/mg of bacterial pellet. The reagent efficiently lysed in 30-60 min and improved protein yield compared with mechanical lysis approaches. It was compatible with downstream purification workflow.
Multiple samples can be lysed in less than hour
Expensive
A very convenient and effective bacterial lysis reagent when protein yield and ease of use are priorities