Thermo Scientific
Zeba Spin Desalting Columns (2mL)
89890
Info:Supplier Page
I am biotinylating and adding a terminal alkyne to a purified protein of interest (17 kDa) using NHS-ester chemistry. I needed to remove the remaining unreacted biotin and alkyne from the in-tact protein via these purification columns.
Protein purification
purified protein, Propargyl-PEG8-NHS, biotin-NHS
The columns must be taped into the tubes!
The columns kept on falling out of the tubes and snapping in the centrifuge, even when the centrifuge was put on a slow ramp up. The only way to fix this was to tape them into the conical collection tubes (photo attached). Even with that, I lost 50% of my protein yield after purification
The columns did remove unreacted biotin and alkyne.
I lost 50% of my protein and the tubes were difficult to work with.
These columns are difficult to use and lose protein, but they will get the job done.