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Author Profile
Vanessa Kelley
Postdoctoral Researcher
The Broad Institute
Articles by Vanessa Kelley
Difficult Protein Purification Columns
August 24, 2026
I am biotinylating and adding a terminal alkyne to a purified protein of interest (17 kDa) using NHS-ester chemistry. I needed to ...
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Strong Fluorophore and Easy Conjugation
August 24, 2026
I needed to label a purified protein of interest with Cy7 using click chemistry. My protein had a terminal alkyne, so I attached ...
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Great Antibody for Total ATM Western Blot
May 01, 2026
We examined whether total ATM levels changed between a wildtype head and neck cancer cell line and a JAK1 knockout cell line. We ...
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Detection of LAP+ Treg Cells by Flow Cytometry
Mitesh Dwivedi
The aim of the experiment was to detect LAP+ CD4+CD25+ regulatory T cells (Tregs) isolated from whole blood of humans.
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