Biolegend
APC anti-human CD274 (B7-H1, PD-L1) Antibody
329707
29E.2A3
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We wanted to confirm PD-L1 surface expression in our A375 human cutaneous melanoma cell line via flow cytometry. To validate, we treated with IFN-gamma, as PD-L1 is a known IFN-gamma target gene.
Flow Cytometry
A375 human cutaneous melanoma
1:400, 30 min 4C
FACS buffer (PBS + 5% FBS + 0.05% Sodium Azide)
N/A
Flow cytometry
Results were as expected- surface expression of PD-L1 was increased after 48 hours IFN-gamma treatment. Analyzed on a Symphony A3 cytometer
Reliable
Easy to validate and will use in future experiments!