Sensitive Residual Protein Quantification After IVT mRNA Production

August 06, 2026

BlueskyReddit
Houston Methodist Research Institute

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Quality of Results

Ease-of-Optimization

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Questions/Comments

Company:

Invitrogen

Product Name:

NanoOrange™ Protein Quantitation Kit

Catalog Number:

N6666 (Kit) / N10272 (Diluent)

I used the NanoOrange™ Protein Quantitation Kit to quantify residual protein following IVT mRNA production. The assay was easy to optimize, highly sensitive for low protein concentrations, and provided reproducible results using a low-range standard curve.

Experimental Design and Results Summary

Application

Residual Protein Quantification

Starting Material

Purified IVT mRNA samples

Tips

A low-range BSA standard curve prepared by serial dilution worked well for quantifying residual protein. During the heating step, incubate samples in PCR tubes or microcentrifuge tubes rather than directly in the microplate to minimize evaporation before transferring to the plate for fluorescence measurement.

Results Summary

The NanoOrange™ assay was used to quantify residual protein after IVT mRNA production. A low-range standard curve generated by serial dilution provided sensitive and reproducible quantification. After heating the samples and transferring them to the microplate, fluorescence measurements produced reliable results with good assay reproducibility.

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Summary

The Good

Excellent sensitivity for low protein concentrations, easy protocol, reproducible results, and ideal for residual protein analysis.

The Bad

The heating step can cause sample evaporation if performed directly in the microplate.

The Bottom Line

A highly sensitive and reliable assay for residual protein quantification. Using a low-range standard curve and heating samples in tubes before plate transfer provides consistent, high-quality results.

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