BaseScope Probe for Npas3 mRNA (Transcription Factor) Detection

July 31, 2026

BlueskyReddit
State University of New York at Buffalo
Physiology and Biophysics
Postdoctoral Researcher

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Company:

Advanced Cell Diagnostics / Bio-Techne

Product Name:

BaseScope™ Probe- BA-Mm-Npas3-3zz-st-C1

Catalog Number:

1186601-C1

We study the basic helix-loop-helix transcription factor neuronal PAS domain protein 3 (NPAS3) and its role in astrocyte energy metabolism. We used a BaseScope Npas3 probe to assess Npas3 mRNA expression in mouse brain tissue.

Experimental Design and Results Summary

Application

BaseScope in situ hybridization

Starting Material

15-μm fixed-frozen mouse brain sections

Tips

We used the Npas3 probe together with the BaseScope Detection Reagent Kit v2-RED (323910, ACD) according to the manufacturer’s instructions (Technical Note MK 51-149/Rev B, ACD) and integrated an immunostaining workflow with the following modification: Protease III (322337, ACD) was used for brain tissue pretreatment during the integrated co-detection workflow.

Results Summary

We used the Npas3 BaseScope probe (1186601-C1, ACD; GenBank accession number NM_013780.3; nucleotides 432–620) to validate the decrease in Npas3 expression in Npas3 conditional knockout mice. In the image: red dots represent Npas3 mRNA transcripts, green represents GFAP immunostaining, and blue represents DAPI staining.

DOI or PMID #

42308317

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Summary

The Good

The probe worked consistently well across multiple experiments and was validated in knockout mice.

The Bad

We did not encounter any issues with this probe.

The Bottom Line

The probe is supplied in a bottle with a sufficient volume for multiple experiments and analysis of numerous brain sections. It is easy to use and consistently provides reliable results across repeated experiments.

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