In situ hybridization (ISH) is a type of histochemical application that capitalizes on sequence-specific DNA or RNA binding.
With the use of labeled, complementary DNA and RNA oligonucleotides, genomic regions and gene expression can be visualized within the context of cells and tissues.
ISH is a well-established technique first introduced in the 1960s to localize the positions of specific DNA sites within a chromosome (Gall and Pardue).
Various labels for ISH probes have been developed and used, including radioactive isotopes and dyes.
Fluorescent in situ hybridization (FISH) makes use of fluorophores, while chromogenic in situ hybridization (CISH) uses probes labeled with biotin or digoxigenin.
These variants of ISH are now widely used in studies in gene amplification, chromosomal analysis, mRNA expression, and microbial detection.
This listing of in situ hybridization products aims to help researchers explore relevant tools, including hybridization probes, ISH kits, reagents, and more.
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In situ hybridization can now be used on a variety of different samples such as morphologically preserved chromosomes, cells, tissue sections, entire tissue, and circulating tumor cells.
This comprehensive guide explores the ins and outs of ISH, as well as its new developments.
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Fluorescent in situ hybridization (FISH) has recently seen a surge in popularity due to advances in oligo synthesis technology and improvements in bioinformatics algorithms.
Moving out of the DNA realm, the capabilities of FISH continue to expand as interest in RNA FISH and transcriptome analysis grows.
Automating single-cell transcriptomic workflows has upended field.
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Tips on ensuring sample quality is maintained
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Introducing the LUNA-FX7™ - the automated cell counter that builds on the success of its predecessors. The LUNA-FX7™ is our most powerful cell counter to date, with unmatched cell counting accuracy, a maximum counting volume of 5 µL (10 times that of...
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The Sapphire FL Biomolecular Imager is a field-upgradable laser scanner, with a wide selection of user-adjustable lasers and filters. It features 5-micron resolution, adjustable Z-plane focus and a wide field of view of 25cm X 25cm. Applications ...
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We study astrocyte bioenergetics and immunometabolism using mouse models. Recently, we found that mice lacking mu opioid receptors in astrocytes exhibit increased mitochondrial metabolism, as well as increased astrocyte reactivity.
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In our experiments, we studied how the transcription factor NPAS3 regulates astrocyte mitochondrial bioenergetics and how this affects the behavioral phenotype of Npas3-deficient mice, with a focus on cognition. We used RNA ISH using the “BaseScope” ...
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