In situ hybridization (ISH) is a type of histochemical application that capitalizes on sequence-specific DNA or RNA binding.
With the use of labeled, complementary DNA and RNA oligonucleotides, genomic regions and gene expression can be visualized within the context of cells and tissues.
ISH is a well-established technique first introduced in the 1960s to localize the positions of specific DNA sites within a chromosome (Gall and Pardue).
Various labels for ISH probes have been developed and used, including radioactive isotopes and dyes.
Fluorescent in situ hybridization (FISH) makes use of fluorophores, while chromogenic in situ hybridization (CISH) uses probes labeled with biotin or digoxigenin.
These variants of ISH are now widely used in studies in gene amplification, chromosomal analysis, mRNA expression, and microbial detection.
This listing of in situ hybridization products aims to help researchers explore relevant tools, including hybridization probes, ISH kits, reagents, and more.
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In situ hybridization can now be used on a variety of different samples such as morphologically preserved chromosomes, cells, tissue sections, entire tissue, and circulating tumor cells.
This comprehensive guide explores the ins and outs of ISH, as well as its new developments.
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Fluorescent in situ hybridization (FISH) has recently seen a surge in popularity due to advances in oligo synthesis technology and improvements in bioinformatics algorithms.
Moving out of the DNA realm, the capabilities of FISH continue to expand as interest in RNA FISH and transcriptome analysis grows.
Common methods for a range of stem cell types
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Interview with Jiye Wang
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The revolutionary MACSima™ Platform streamlines your spatial biology research from end to end, reducing experimental workload and generating impactful data in no time. At its core is the fully automated MACSima™ System, which utilizes fluorescence ...
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Use TissueFAXS Spectra for whole-slide multispectral imaging as well as high-end image analysis. This system allows the scanning of up to 8 markers in one run and thereby is an ideal solution for in-depth phenotyping. A liquid crystal tunable filter ...
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We study astrocyte bioenergetics and immunometabolism using mouse models. Recently, we found that mice lacking mu opioid receptors in astrocytes exhibit increased mitochondrial metabolism, as well as increased astrocyte reactivity.
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In our experiments, we studied how the transcription factor NPAS3 regulates astrocyte mitochondrial bioenergetics and how this affects the behavioral phenotype of Npas3-deficient mice, with a focus on cognition. We used RNA ISH using the “BaseScope” ...
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