Bright and Clean Nuclear Staining with Convenient Mounting Medium

Stanford University
Department of Pathology
Postdoctoral Researcher

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Questions/Comments

Company:

Biotium

Product Name:

EverBrite™ Hardset Mounting Medium with NucSpot® 640

Catalog Number:

23016

I use fluorescence microscopy to analyze cellular localization. I was looking for a replacement of DAPI for staining nucleus when I am working with BFP tagged proteins. This mounting media contains nuclear stain that can be detected in the red channel.

Experimental Design and Results Summary

Application

Immunofluorescence microscopy for nuclear counterstaining

Starting Material

Fixed mammalian cells prepared for fluorescence imaging

Tips

Aliquot for longer stability and storage.

Results Summary

This mounting medium provides a simple way to preserve samples while producing strong, clean nuclear staining.

Image Gallery

Summary

The Good

Bright signal, clean background, and convenient all-in-one mounting solution.

The Bad

NA

The Bottom Line

A reliable and convenient mounting medium for fluorescence imaging that provides excellent nuclear contrast.

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