Difficult Optimization but Detectable Outcome

July 23, 2026

BlueskyReddit
Cell & Tissue Engineering, Imec
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Glucose Transporter GLUT1 antibody [EPR3915]

Catalog Number:

ab115730

Clone Number:

Info:Supplier Page View Product Specs

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GLUT1 strongly labels brain microvascular endothelial cells (BMECs), making it useful for visualizing the cerebral vasculature. We used this antibody to detect BMECs in samples obtained from rats.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Formalin-fixed Paraffin-embedded (FFPE), rat samples (4 um)

Primary Incubation

Dilute to 1:300 in NGS/BSA, 1h @ RT; Samples were previously exposed to 1% Triton X-100

Blocking Agent

Normal goat serum diluted in bovine serum albumin (4%)

Secondary Incubation

AlexaFluor 555 goat anti-rabbit IgG (#A11034, Invitrogen) or Alexa Fluor 488 goat anti-rabbit (#A11034, Invitrogen), 1:1000

Tertiary Incubation

none

Detection

Confocal, fluorescent microscope

Results Summary

This antibody performs well both as a standalone marker and in combination with other assays, such as the ACD co-detection kit for RNAscope® in situ hybridization (as shown in the image). This antibody provides clear labeling of both large vessels and the capillary network.

DOI or PMID #

https://doi.org/10.1016/j.jconrel.2026.115174

Additional Notes

Although BBB endothelial cells show the strongest signal, GLUT1 is also expressed in the choroid plexus epithelium, or in some glial compartments.

Image Gallery

Summary

The Good

Great detection using a high dilution

The Bad

None

The Bottom Line

The Anti-GLUT1 antibody is an excellent choice for visualizing the cerebral vasculature in rat brain, providing robust labeling of both major vessels and the capillary network. Its high specificity, compatibility with multiplex assays such as RNAscope®, and widespread validation make it a reliable marker for studies of brain vascular architecture and the blood–brain barrier. However, because GLUT1 expression can vary with disease state, it is best interpreted as a BBB endothelial marker rather than a universal indicator of vascular abundance.

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