Cell Signaling Technologies
CYR61 Rabbit Monoclonal Antibody
#39382
E5W3H
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We've created a TAZ overexpression system in murine melanoma cells. To validate downstream TEAD targets, we use this Cyr61 antibody which has validated wonderfully. We've also used in human melanoma cell lines and had great results as well.
Western Blot
1014 murine melanoma cells
1:100, o/n at 4C
5% BSA
1:4000, 1 hour at room temperature
N/A
Chemiluminescence on Biorad ChemiDoc
Used to validate TEAD transciptional activity as downstream target. Works well in multiple mouse and human melanoma cell lines in different contexts. (TAZ overexpression, TAZ knockdown, TEAD inhibitor treatment etc). Also used for lysates taken from in vivo tumors.
41193428
Great signal, reproducible results
Highly recommend if wanting to validate TEAD target expression