Bio-Rad
Criterion TGX Stain-Free™ 18-well protein gel
5678094
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My research focuses on understanding the function of a bacterial cell wall hydrolase and its localization in Enterococcus using confocal microscopy. To achieve this, I constructed both N- and C-terminal HaloTag fusion proteins. Before imaging, I used western blotting to evaluate the expression and stability of the fusion proteins. The Criterion TGX Stain-Free™ 18-Well Protein Gel was ideal for these experiments, allowing me to load larger sample volumes, which was especially important because the C-terminal HaloTag fusion showed relatively low expression.
SDS-PAGE protein separation followed by western blot
exponentially-grown bacterial cell lysates
Avoid overloading in the wells. Also, the gel should be treated carefully as it is prone to breaking. Make sure the gel remains properly wet to maintain its integrity during handling.
The stain-free gel was highly convenient because it eliminated the need for additional Coomassie blue staining. The protein separation was excellent, and I was able to clearly visualize my protein bands directly on the gel before proceeding to western blot analysis. The strong visibility was likely due to good protein expression in my system, but the gel itself provided excellent resolution and allowed quick assessment of protein levels and sample quality.
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Sharp separation and excellent consistency
The gels are more expensive than standard gels
A dependable gel that saves time while producing high-quality protein separation.