High-Quality CleanCap® EGFP mRNA for HEK293 Transfection Studies

July 01, 2026

BlueskyReddit
University of Florida
Neurosurgery
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Questions/Comments

Company:

TriLink BioTechnologies

Product Name:

CleanCap® EGFP mRNA

Catalog Number:

L-7601-1000

I used CleanCap® EGFP mRNA to evaluate DOTAP-based lipid nanoparticle formulations in HEK293 cells. The mRNA produced strong EGFP expression detected by flow cytometry in the FITC channel, and the supplied concentration of 1 mg/mL was confirmed by NanoDrop before use.

Experimental Design and Results Summary

Application

Evaluation of mRNA delivery and protein expression in HEK293 cells using DOTAP lipid nanoparticle formulations.

Starting Material

Tips

The mRNA concentration was confirmed by NanoDrop (1 mg/mL) before formulation. Multiple mRNA doses were tested in HEK293 cells using DOTAP formulations to optimize transfection efficiency. EGFP expression was quantified by flow cytometry using the FITC channel.

Results Summary

The CleanCap® EGFP mRNA generated strong GFP expression following DOTAP-mediated delivery into HEK293 cells. Flow cytometry showed robust fluorescence in the FITC channel across the optimized formulations, demonstrating efficient translation and excellent mRNA quality. NanoDrop analysis confirmed the supplied concentration of 1 mg/mL, providing confidence in formulation preparation and dose calculations.

DOI or PMID #

N/A

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Summary

The Good

Excellent mRNA quality and integrity

The Bad

No significant drawbacks were observed

The Bottom Line

TriLink CleanCap® EGFP mRNA is an excellent reagent for evaluating mRNA delivery systems. It consistently produced strong GFP expression in HEK293 cells after DOTAP-mediated transfection, and the verified 1 mg/mL concentration enabled accurate dosing and reproducible flow cytometry results. It is highly recommended for optimizing mRNA-LNP and lipoplex formulations.

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