
The non-viral transfection of genetic material into cells, also known as gene delivery, is simplified by commercially available reagents and kits. The appropriate method for gene delivery depends on the type of cell and genetic material. Plasmid DNA, siRNA, proteins, and antibodies are transfected into cells using methods such as liposomes, calcium phosphate, and electroporation. Proficient stable or transient cell lines are generated using established cell lines, primary cells or stem cells with the appropriate transfection method. In vivo transfection reagents are offered with markers, such as Neomycin resistance. In addition to basic transfection kits, enhancement compounds can be used to increase the efficiency of the selected transfection treatment. A diverse range of transfection products is available to match the required gene delivery method.
Insights from research presented at a recent Biocompare webinar
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WES not likely to be entirely replaced by WGS any time soon.
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The MilliSentials™ Lab Labeling System provides a complete laboratory labeling solution with laboratory grade labels, a compact WIFI capable printer, and custom-designed laboratory labeling software. Features:
Laboratory grade labels
Labels can ...
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Hold your research and proof of concept studies to the highest standards with reliable and efficient electroporation technology. Features:
Achieve high efficiency and cell viability
Rapidly transfect from 75 thousand to 700 million cells
...
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I often transiently overexpress proteins in U2OS cells and found Lipofectamine 3000 to be the best transfection reagent for this cell line.
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Lipofectamine 3000 Transfection Reagent gives good transfection performance with improved application outcomes and reproducible results.
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