The non-viral transfection of genetic material into cells, also known as gene delivery, is simplified by commercially available reagents and kits. The appropriate method for gene delivery depends on the type of cell and genetic material. Plasmid DNA, siRNA, proteins, and antibodies are transfected into cells using methods such as liposomes, calcium phosphate, and electroporation. Proficient stable or transient cell lines are generated using established cell lines, primary cells or stem cells with the appropriate transfection method. In vivo transfection reagents are offered with markers, such as Neomycin resistance. In addition to basic transfection kits, enhancement compounds can be used to increase the efficiency of the selected transfection treatment. A diverse range of transfection products is available to match the required gene delivery method.
Tech is helping usher in a new era of personalized cancer treatment
read more
Tips to help you overcome data integration & analysis challenges
read more
Choose the CFX Opus real-time PCR system for the reliability you depend on from Bio-Rad with improved connectivity and performance for your modern lab. The CFX Opus system is the next evolution in qPCR from Bio-Rad. With improved thermal performance ...
read more
Speed and enhanced PCR optimization functions like the 2D-Gradient make the Mastercycler X50 the ideal PCR cycler for advanced research in molecular biology. The excellent block temperature regulation gives rise to the next stage of PCR ...
read more
The Neon Transfection System 100 µL Kit was used with the Neon Transfection System to deliver siRNA into primary immune cells, and the results have been excellent and highly reproducible.
read more
Lipofectamine has been my default choice whenever transfection efficiency is critical. It consistently delivers strong expression levels with straightforward protocols and minimal troubleshooting in commonly used cell lines. The main drawback is cost...
read more