This Antibody May Not Recognize the UNC93A Protein Under Tested Conditions

June 12, 2026

BlueskyReddit
Health Division of Gastroenterology
UC Davis

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Company:

Sigma

Product Name:

Anti-UNC93A antibody produced in rabbit

Catalog Number:

ATL-HPA035729

Clone Number:

N/A

Info:Supplier Page

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We generated an UNC93A overexpression construct and transiently transfected it into HEK293T cells. While the FLAG-tagged UNC93A protein was readily detected using an anti-FLAG antibody, we were unable to detect a corresponding band using the tested UNC93A antibody. These results suggest that the overexpression construct is expressed successfully, but the antibody does not recognize UNC93A under Western blot conditions even with 1:100 dilution.

Experimental Design and Results Summary

Applications

Western blot

Sample

HEK293T cells

Primary Incubation

1:100 dilution, overnight

Blocking Agent

3% BSA in PBS

Secondary Incubation

1:1000 goat anti-rabbit

Tertiary Incubation

1hr incubation at room temp

Detection

LICOR fluorescence

Results Summary

We generated an UNC93A overexpression construct and transiently transfected it into HEK293T cells. While the FLAG-tagged UNC93A protein was readily detected using an anti-FLAG antibody, we were unable to detect a corresponding band using the tested UNC93A antibody. These results suggest that the overexpression construct is expressed successfully, but the antibody does not recognize UNC93A under Western blot conditions even with 1:100 dilution.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

NA

The Bad

Did not work.

The Bottom Line

This antibody does not recognize the UNC93A protein.

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