Trusted Background Staining With Rat IgG2a ? Isotype Control

June 11, 2026

BlueskyReddit
Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

ThermoFisher Scientific

Product Name:

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Catalog Number:

12-4321-81

Clone Number:

N/A

Info: Supplier Page 

This isotype control was used during flow cytometric analysis of mouse tumor samples to assess B7-H3 expression. When the anti-B7-H3 antibody generated minimal signal, the isotype control helped confirm that the observed staining profile reflected true background levels rather than nonspecific antibody binding, supporting the interpretation of the negative result.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Murine tumor cells

Primary Incubation

30 minutes

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

The Rat IgG2a, κ Isotype Control provided a reliable negative control for flow cytometry experiments, helping confirm that observed staining patterns were specific to the target antibody rather than nonspecific background signal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to incorporate into staining workflows, produced consistently low background, and enabled clear validation of antibody specificity.

The Bad

As with any isotype control, proper matching to the experimental antibody and staining conditions is required for meaningful interpretation.

The Bottom Line

A dependable and straightforward isotype control.

Share your experience with other scientists. Write a Review! »

Join the discussion