Great results when optimized, optimization takes awhile

Inhibrx
Cell Bio
Research Scientist

Overall

Performance

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Bio-Rad

Product Name:

QX600 Droplet Digital PCR System

Catalog Number:

17007769

My lab uses the QX600 system for assessing gene copy number in human cells. We run a wide variety of sample types, but predominantly human iPSC DNA or cDNA.

Experimental Design and Results Summary

Application

ddPCR

Tips

N/A

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Once optimized, the QX600 system is capable of intensive multiplexing that no other digital PCR machine on the market can match. Some of our assays can asses the copy number of over 6 different genes per well.

The Bad

Getting the hang of generating perfect droplets is time-intensive. Once you have your own handling optimized, the droplet generator machine itself is also quite prone to suddenly not working. I think this year we have had to have parts replaced in the generator 3 different times.

The Bottom Line

This system has great sensitivity and intense multiplexing capabilities, but it is hard to optimize. Even once you finally have everything ironed out, the droplet generator machine itself is sort of unreliable, breaking multiple times per year (with admittedly very high volume usage though).

Join the discussion