BioLegend Anti-CD86: Superior Resolution and Reliability

Medicine
Weill Cornell Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cyanine7 anti-mouse CD86 Antibody

Catalog Number:

105013

Clone Number:

GL-1

This antibody performed exceptionally well in our studies, demonstrating strong specificity, consistent staining, and excellent reproducibility across experiments.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestinal lamina propria lymphocytes

Primary Incubation

1:200 for 20 minutes at 4°C

Blocking Agent

Anti-CD16/CD32 Fc receptor blocking antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

The antibody demonstrated excellent specificity, sensitivity, and reproducibility in murine immune cell populations.

DOI or PMID #

38867048

Additional Notes

The antibody was validated in multiple tissues and experimental conditions and showed robust performance with standard flow cytometry staining protocols.

Related Categories

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Summary

The Good

Excellent clear population resolution, and highly reproducible staining across experiments.

The Bad

No major issues encountered.

The Bottom Line

A reliable, high-quality antibody that provides consistent and reproducible results for flow cytometric analysis. Highly recommended for immunological studies.

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